Independent mis‐splicing mutations in TaPHS1 causing loss of preharvest sprouting (PHS) resistance during wheat domestication. Issue 3 (10th August 2015)

• Record Type: Journal Article
• Authors: Liu, Shubing; Sehgal, Sunish K.; Lin, Meng; Li, Jiarui; Trick, Harold N.; Gill, Bikram S.; Bai, Guihua
• Is Part Of: New phytologist. Volume 208:Issue 3(2015:Dec. 01)
• Page Start: 928
• Page End: 935
• Publication Date: 2015-08-10
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.13489
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph13489-abs-0001"> <title>Summary</title> <p> <list id="nph13489-list-0001" list-type="bullet"> <list-item> <p>Preharvest sprouting (PHS) is one of the major constraints of wheat production in areas where prolonged rainfall occurs during harvest. <italic>TaPHS1</italic> is a gene that regulates PHS resistance on chromosome 3A of wheat, and two causal mutations in the positions +646 and +666 of the <italic>TaPHS1</italic> coding region result in wheat PHS susceptibility. Three competitive allele‐specific PCR (KASP) markers were developed based on the two mutations in the coding region and one in the promoter region and validated in 82 wheat cultivars with known genotypes. These markers can be used to transfer <italic>TaPHS1</italic> in breeding through marker‐assisted selection.</p> </list-item> <list-item> <p>Screening of 327 accessions of wheat A genome progenitors using the three KASP markers identified different haplotypes in both diploid and tetraploid wheats. Only one <italic>Triticum monococcum</italic> accession, however, carries both causal mutations in the <italic>TaPHS1</italic> coding region and shows PHS susceptibility. Five of 249 common wheat landraces collected from the Fertile Crescent and surrounding areas carried the mutation (C) in the promoter (−222), and one landrace carries both the causal mutations in the <italic>TaPHS1</italic> coding region, indicating that the mis‐splicing (+646) mutation occurred during common wheat domestication.</p> </list-item> <list-item> <p>PHS assay of wheat progenitor accessions demonstrated that the wild‐types were highly PHS‐resistant, whereas the domesticated type showed increased PHS susceptibility.</p> </list-item> <list-item> <p>The mis‐splicing <italic>TaPHS1</italic> mutation for PHS susceptibility was involved in wheat domestication and might arise independently between <italic>T. monococcum</italic> and <italic>Triticum aestivum</italic>.</p> </list-item> </list> </p> </abstract>