Antifungal effect of gamma irradiation and sodium dichloroisocyanurate against Penicillium expansum on pears. (24th September 2015)

• Record Type: Journal Article
• Authors: Jeong, R.‐D.; Chu, E.‐H.; Shin, E.‐J.; Lee, E.‐S.; Kwak, Y.‐S.; Park, H.‐J.
• Is Part Of: Letters in applied microbiology. Volume 61:Number 5(2015:Nov.)
• Page Start: 437
• Page End: 445
• Publication Date: 2015-09-24
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12466
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" id="lam12466-abs-0001"> <title>Abstract</title> <sec id="lam12466-sec-1011" sec-type="section"> <p>Gamma irradiation (GI) was evaluated for its <italic>in vitro</italic> and <italic>in vivo</italic> antifungal activity against <italic>Penicillium expansum</italic> on pear fruits. GI showed a complete inhibition of spore germination, germ tube elongation and mycelial of <italic>P. expansum</italic>, especially 1·8 kGy. GI affected the membrane integrity and cellular leakage of conidia in a dose‐dependent manner. Furthermore, the leakage of protein and sugar from mycelia increased along with the dose. GI was evaluated at lower doses in combination with a chlorine donor, sodium dichloro‐<italic>s</italic>‐triazinetrione (NaDCC), to examine the inhibition of <italic>P. expansum</italic>. Interestingly, only a combined treatment with 0·2 kGy of GI and 70 ppm of NaDCC exhibited significant synergistic antifungal activity. The mechanisms by which the combined treatment decreased the blue mould decay of pear fruits could directly associated with the disruption of the cell membrane of the fungal pathogen, resulting in a loss of cytoplasmic materials from the hyphae.</p> </sec> <sec id="lam12481-sec-1012" sec-type="section"> <title>Significance and Impact of the Study</title> <p>Gamma irradiation (GI) is used as an effective nonchemical approach to inactive pathogens. This study investigated the antifungal effect of gamma irradiation and its combined treatment with a chlorine donor on this fungal pathogen, both <italic>in vitro</italic> and <italic>in vivo</italic>. This study emphasized that the integration of low‐dose GI and a chlorine donor, NaDCC, exhibited a significant antifungal effect, and that its mechanisms are directly associated with membrane integrity of fungal spores, promising that GI has the potential to be an antifungal approach.</p> </sec> </abstract>