Exploring the expression of Pseudomonas aeruginosa genes directly from sputa of cystic fibrosis patients. (21st September 2015)

• Record Type: Journal Article
• Authors: Barthe, C.; Nandakumar, S.; Derlich, L.; Macey, J.; Bui, S.; Fayon, M.; Crouzet, M.; Garbay, B.; Vilain, S.; Costaglioli, P.
• Is Part Of: Letters in applied microbiology. Volume 61:Number 5(2015:Nov.)
• Page Start: 423
• Page End: 428
• Publication Date: 2015-09-21
• Subjects: Microbiology -- Periodicals; 660.62
• DOI: 10.1111/lam.12465
• Languages: English
• ISSNs: 0266-8254

Abstract

<abstract abstract-type="main" id="lam12465-abs-0001"> <title>Abstract</title> <sec id="lam12465-sec-1011" sec-type="section"> <p>Acquisition of <italic>Pseudomonas aeruginosa</italic> is known as a negative prognostic factor in patients with cystic fibrosis. We started a pilot study to evaluate <italic>Ps. aeruginosa</italic> gene expression directly from the sputum of infected patients. Total RNA was purified from 15 sputum samples collected from 10 patients, and the expression levels of five genes from <italic>Ps. aeruginosa</italic> were measured by RT‐qPCR. Expression of <italic>algD</italic>, <italic> algR, antB</italic>, <italic> lasB and pqsA</italic> genes was determined in sputa that contained <italic>Ps. aeruginosa</italic> cells. The resultant data provided an overview of the expression of these genes in CF patients. Except for the correlation between <italic>algD</italic> expression and the mucoid phenotype, the gene expression profile could not be associated with the clinical status of patients. However, beyond the heterogeneity of the <italic>Ps. aeruginosa</italic> phenotype in sputum, we observed a correlation between the expression of <italic>antB</italic> and <italic>pqsA</italic> and a low level of <italic>lasB</italic> transcripts.</p> </sec> <sec id="lam12465-sec-1012" sec-type="section"> <title>Significance and Impact of the Study</title> <p> <italic>Pseudomonas aeruginosa</italic> infection leads to high morbidity and mortality in cystic fibrosis patients. The identification of <italic>Ps. aeruginosa</italic>‐assigned factors is important to eradicate the colonization. We started a pilot study to evaluate the gene expression of <italic>Ps. aeruginosa</italic> directly from the sputum of infected patients. Preliminary results suggest that beyond the heterogeneity of the <italic>Ps. aeruginosa</italic> phenotype in sputum, we observe a correlation between the expression of <italic>antB</italic> and <italic>pqsA</italic> and a low level of <italic>lasB</italic> transcripts. This approach could shed some light on the behaviour of <italic>Ps. aeruginosa</italic> during pulmonary infection and may reveal some important elements for optimizing therapy.</p> </sec> </abstract>