Protection against Paracoccidioides brasiliensis infection in mice treated with modulated dendritic cells relies on inhibition of interleukin‐10 production by CD8+ T cells. Issue 3 (21st September 2015)

• Record Type: Journal Article
• Authors: Alves da Costa, Thiago; Di Gangi, Rosária; Martins, Paula; Longhini, Ana Leda Figueiredo; Zanucoli, Fábio; de Oliveira, Alexandre Leite Rodrigues; Stach‐Machado, Dagmar Ruth; Burger, Eva; Verinaud, Liana; Thomé, Rodolfo
• Is Part Of: Immunology. Volume 146:Issue 3(2015:Nov.)
• Page Start: 486
• Page End: 495
• Publication Date: 2015-09-21
• Subjects: Immunology -- Periodicals
• DOI: 10.1111/imm.12526
• Languages: English
• ISSNs: 0019-2805

Abstract

<abstract abstract-type="main" id="imm12526-abs-0001"> <title>Summary</title> <p>Paracoccidioidomycosis is a systemic infection prevalent in Latin American countries. Disease develops after inhalation of <italic>Paracoccidioides brasiliensis</italic> conidia followed by an improper immune activation by the host leucocytes. Dendritic cells (DCs) are antigen‐presenting cells with the unique ability to direct the adaptive immune response by the time of activation of naive T cells. This study was conducted to test whether extracts of <italic>P. brasiliensis</italic> would induce maturation of DCs. We found that DCs treated with extracts acquired an inflammatory phenotype and upon adoptive transfer conferred protection to infection. Interestingly, interleukin‐10 production by CD8⁺ T cells was ablated following DC transfer. Further analyses showed that lymphocytes from infected mice were high producers of interleukin‐10, with CD8⁺ T cells being the main source. Blockage of cross‐presentation to CD8⁺ T cells by modulated DCs abolished the protective effect of adoptive transfer. Collectively, our data show that adoptive transfer of <italic>P. brasiliensis</italic>‐modulated DCs is an interesting approach for the control of infection in paracoccidioidomycosis.</p> </abstract>