In vitro evaluation of a novel biofilm remover. Issue 4 (24th November 2014)

• Record Type: Journal Article
• Authors: Hofer, D; Meier, A; Sener, B; Guggenheim, B; Attin, T; Schmidlin, PR
• Is Part Of: International journal of dental hygiene. Volume 13:Issue 4(2015:Nov.)
• Page Start: 246
• Page End: 253
• Publication Date: 2014-11-24
• Subjects: Dental hygiene -- Periodicals; Dental hygienists -- Periodicals; 617.60105
• DOI: 10.1111/idh.12113
• Languages: English
• ISSNs: 1601-5029

Abstract

<abstract abstract-type="main" id="idh12113-abs-0001"> <title>Abstract</title> <sec id="idh12113-sec-0001" sec-type="section"> <title>Objective</title> <p>To evaluate a novel device for its efficacy in removing experimental biofilm from root surfaces and its potential for concomitantly removing/roughening the surface substance.</p> </sec> <sec id="idh12113-sec-0002" sec-type="section"> <title>Methods and Materials</title> <p>A novel acrylic rotary device (biofilm remover, BR) was tested <italic>in vitro</italic> in three experiments: surface loss, surface roughness [positive controls: Perioset (PS) and Proxoshape (PR)] and biofilm removal [positive controls: ultrasonic (US) and PS]. Surface loss/surface roughness was evaluated for dentin samples instrumented for three 20 s periods. The calcium removed during instrumentation was analysed after each interval and cumulatively, using atomic absorption spectrophotometry (AAS). Surface roughness was measured using profilometric analysis. Biofilm removal was evaluated on dentin specimens coated with a 64.5 h 6‐species <italic>in vitro</italic> formed biofilm, after one 20 s treatment. Surface loss was analysed using <sc>anova</sc> with Scheffé <italic>post hoc</italic> test, and surface roughness/biofilm removal was analysed using Mann–Whitney test (all <italic>P</italic> ≤ 0.05).</p> </sec> <sec id="idh12113-sec-0003" sec-type="section"> <title>Results</title> <p>Significantly less substance loss [μg (±1 SD)] was observed with the novel device at all time points, both interval and cumulative (1.0 (±0.5) versus 9.3 (±3.2) PS and 9.9 (±1.9) PR at 60 s). Surface roughness [μm (95% CI)] was significantly lower for BR than for PS and PR [0.00 (−0.01, 0.08) 0.20 (0.16, 0.27) and 0.21 (0.19, 0.24) at 60 s]. Significantly less biofilm bacteria remained after treatment with both BR 4.5 (−0.1, 16.2) and US 1.9 (−0.2, 14.3), compared to PS 52 (27.9, 82.1).</p> </sec> <sec id="idh12113-sec-0004" sec-type="section"> <title>Conclusions</title> <p>The novel biofilm remover was less damaging to dentin surfaces, while removing biofilm at least as effectively as devices used in this study.</p> </sec> </abstract>