Peri‐implant bone organization surrounding zirconia‐microgrooved surfaces circularly polarized light and confocal laser scanning microscopy study. Issue 11 (21st August 2014)

• Record Type: Journal Article
• Authors: Delgado‐Ruiz, Rafael Arcesio; Abboud, Marcus; Romanos, Georgios; Aguilar‐Salvatierra, Antonio; Gomez‐Moreno, Gerardo; Calvo‐Guirado, Jose Luis
• Is Part Of: Clinical oral implants research. Volume 26:Issue 11(2015:Nov.)
• Page Start: 1328
• Page End: 1337
• Publication Date: 2014-08-21
• Subjects: Dental implants -- Research -- Periodicals; 617.69
• DOI: 10.1111/clr.12461
• Languages: English
• ISSNs: 0905-7161

Abstract

<abstract abstract-type="main" id="clr12461-abs-0001"> <title>Abstract</title> <sec id="clr12461-sec-0001" sec-type="section"> <title>Objectives</title> <p>To study the peri‐implant bone organization pattern of immediately loaded (IL) zirconia implants with microgrooved surfaces.</p> </sec> <sec id="clr12461-sec-0002" sec-type="section"> <title>Materials and Methods</title> <p>Forty‐eight dental implants of 4 mm diameter and 10 mm length were inserted after two months postextraction healing in the edentulous mandible of six dogs. Three groups of sixteen implants were used, titanium implants (Control), zirconia implants (test A), and zirconia‐microgrooved implants (test B), which were loaded immediately. After 4‐month healing period, implant–bone samples were processed and analyzed by circularly polarized light (CPL) and confocal laser scanning microscopy (CLSM) in two regions of interest ROI<sub>1</sub> (to evaluate the interthread bone) and ROI<sub>2</sub> (to evaluate the bone adjacent to the threads) of 1 mm thickness × 10 mm length each one.</p> </sec> <sec id="clr12461-sec-0003" sec-type="section"> <title>Results</title> <p>Bone organization differs near to the test B, compared with test A and control surfaces, active remodeling was detected surrounding test B implants, with alternancy of organized zones, meanwhile controls and test A areas showed organized areas mainly at 2 mm of implant surfaces. Transverse collagen fibers were significatively higher at ROI<sub>1</sub> for test B implants (60.34 ± 4.34%), compared with controls (47.25 ± 3.51%) and test A (43.78 ± 2.78%) groups (<italic>P</italic> < 0.05). Meanwhile, it was not found any significant difference between groups in ROI<sub>2</sub> (<italic>P</italic> > 0.05). CLSM confirmed the presence of collagen mineralized matrix inside microgrooves of test B groups. 3D reconstruction showed blood vessels in direct contact with the implant surfaces of all groups and bone and blood vessels penetration inside the microgrooves in test B group.</p> </sec> <sec id="clr12461-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The organized pattern of the microgrooved surfaces is able to induce transverse collagen fiber microenvironment reaction to the load, being positive to promote and to maintain the bone remodeling; in addition blood vessels and bone cells are able to penetrate microgrooved surfaces.</p> </sec> </abstract>