A Redox Hydrogel Protects the O2‐Sensitive [FeFe]‐Hydrogenase from Chlamydomonas reinhardtii from Oxidative Damage1. Issue 42 (12th June 2015)

• Record Type: Journal Article
• Authors: Oughli, Alaa Alsheikh; Conzuelo, Felipe; Winkler, Martin; Happe, Thomas; Lubitz, Wolfgang; Schuhmann, Wolfgang; Rüdiger, Olaf; Plumeré, Nicolas
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 42(2015)
• Page Start: 12329
• Page End: 12333
• Publication Date: 2015-06-12
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201502776
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>The integration of sensitive catalysts in redox matrices opens up the possibility for their protection from deactivating molecules such as O₂. [FeFe]‐hydrogenases are enzymes catalyzing H₂ oxidation/production which are irreversibly deactivated by O₂. Therefore, their use under aerobic conditions has never been achieved. Integration of such hydrogenases in viologen‐modified hydrogel films allows the enzyme to maintain catalytic current for H₂ oxidation in the presence of O₂, demonstrating a protection mechanism independent of reactivation processes. Within the hydrogel, electrons from the hydrogenase‐catalyzed H₂ oxidation are shuttled to the hydrogel–solution interface for O₂ reduction. Hence, the harmful O₂ molecules do not reach the hydrogenase. We illustrate the potential applications of this protection concept with a biofuel cell under H₂/O₂ mixed feed.</p> </abstract>