Decreased expression of lysophosphatidylcholine (16:0/OH) in high resolution imaging mass spectrometry independently predicts biochemical recurrence after surgical treatment for prostate cancer. Issue 16 (1st September 2015)

• Record Type: Journal Article
• Authors: Goto, Takayuki; Terada, Naoki; Inoue, Takahiro; Kobayashi, Takashi; Nakayama, Kenji; Okada, Yoshiyuki; Yoshikawa, Takeshi; Miyazaki, Yu; Uegaki, Masayuki; Utsunomiya, Noriaki; Makino, Yuki; Sumiyoshi, Shinji; Yamasaki, Toshinari; Kamba, Tomomi; Ogawa, Osamu
• Is Part Of: Prostate. Volume 75:Issue 16(2015)
• Page Start: 1821
• Page End: 1830
• Publication Date: 2015-09-01
• Subjects: Prostate -- Diseases -- Periodicals; 616
• DOI: 10.1002/pros.23088
• Languages: English
• ISSNs: 0270-4137

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="pros23088-sec-0001" sec-type="section"> <title>Background</title> <p>Human prostate cancers are highly heterogeneous, indicating a need for various novel biomarkers to predict their prognosis. Lipid metabolism affects numerous cellular processes, including cell growth, proliferation, differentiation, and motility. Direct profiling of lipids in tissue using high‐resolution matrix‐assisted laser desorption/ionization imaging mass spectrometry (HR‐MALDI‐IMS) may provide molecular details that supplement tissue morphology.</p> </sec> <sec id="pros23088-sec-0002" sec-type="section"> <title>Methods</title> <p>Prostate tissue samples were obtained from 31 patients, with localized prostate cancer who underwent radical prostatectomy. The samples were assessed by HR‐MALDI‐IMS in positive mode, with the molecules identified by tandem mass spectrometry (MS/MS). The effect of identified molecules on prostate specific antigen recurrence free survival after radical prostatectomy was determined by Cox regression analysis and by the Kaplan–Meier method.</p> </sec> <sec id="pros23088-sec-0003" sec-type="section"> <title>Results</title> <p>Thirteen molecules were found to be highly expressed in prostate tissue, with five being significantly lower in cancer tissue than in benign epithelium. MS/MS showed that these molecules were [lysophosphatidylcholine (LPC)(16:0/OH)+H]<sup>+</sup>, [LPC(16:0/OH)+Na]<sup>+</sup>, [LPC(16:0/OH)+K]<sup>+</sup>, [LPC(16:0/OH)+matrix+H]<sup>+</sup>, and [sphingomyelin (SM)(d18:1/16:0)+H]<sup>+</sup>. Reduced expression of LPC(16:0/OH) in cancer tissue was an independent predictor of biochemical recurrence after radical prostatectomy.</p> </sec> <sec id="pros23088-sec-0004" sec-type="section"> <title>Conclusions</title> <p>HR‐MALDI‐IMS showed that the expression of LPC(16:0/OH) and SM(d18:1/16:0) was lower in prostate cancer than in benign prostate epithelium. These differences in expression of phospholipids may predict prostate cancer aggressiveness, and provide new insights into lipid metabolism in prostate cancer. <italic>Prostate 75:1821–1830, 2015</italic>. © 2015 Wiley Periodicals, Inc.</p> </sec> </abstract>