"You produce while I clean up", a strategy revealed by exoproteomics during Synechococcus–Roseobacter interactions. Issue 20 (21st April 2015)

• Record Type: Journal Article
• Authors: Christie‐Oleza, Joseph A.; Scanlan, David J.; Armengaud, Jean; Jagtap, Pratik; Griffin, Tim; Armengaud, Jean
• Is Part Of: Proteomics. Volume 15:Issue 20(2015:Oct.)
• Page Start: 3454
• Page End: 3462
• Publication Date: 2015-04-21
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201400562
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro‐organisms. However, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance of marine microbiomes that ultimately feed these higher trophic levels. The primary produced organic matter is mostly remineralized by heterotrophic microorganisms but, because most of the oceanic dissolved organic matter is in the form of biopolymers, and microbial membrane transport systems operate with molecules <0.6 kDa, it must be hydrolyzed outside the cell before a microorganism can acquire it. As a <italic>simili</italic> of the marine microbiome, we analyzed, using state‐of‐the‐art proteomics, the exoproteomes obtained from synthetic communities combining specific <italic>Roseobacter</italic> (<italic>Ruegeria pomeroyi</italic> DSS‐3, <italic>Roseobacter denitrificans</italic> OCh114, and <italic>Dinoroseobacter shibae</italic> DFL‐12) and <italic>Synechococcus</italic> strains (WH7803 and WH8102). This approach identified the repertoire of hydrolytic enzymes secreted by <italic>Roseobacter</italic>, opening up the black box of heterotrophic transformation/remineralization of biopolymers generated by marine phytoplankton. As well as highlighting interesting exoenzymes this strategy also allowed us to infer clues on the molecular basis of niche partitioning.</p> </abstract>