Alpinia katsumadai Extracts Inhibit Adhesion and Invasion of Campylobacter jejuni in Animal and Human Foetal Small Intestine Cell Lines. (9th June 2015)

• Record Type: Journal Article
• Authors: Pogačar, Maja Šikić; Klančnik, Anja; Bucar, Franz; Langerholc, Tomaž; Možina, Sonja Smole
• Is Part Of: Phytotherapy research. Volume 29:Number 10(2015:Oct.)
• Page Start: 1585
• Page End: 1589
• Publication Date: 2015-06-09
• Subjects: Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.5396
• Languages: English
• ISSNs: 0951-418X

Abstract

<abstract abstract-type="main" id="ptr5396-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Alpinia katsumadai</italic> is used in traditional Chinese medicine for abdominal distention, pain, and diarrhoea. <italic>Campylobacter jejuni</italic> is the most common cause of bacterial food‐borne diarrhoeal illnesses worldwide. Adhesion to gut epithelium is a prerequisite in its pathogenesis. The antimicrobial, cytotoxic, and anti‐adhesive activities of a chemically characterised extract (SEE) and its residual material of hydrodistillation (hdSEE‐R) from <italic>A. katsumadai</italic> seeds were evaluated against <italic>C. jejuni</italic>. Minimal inhibitory concentrations for SEE and hdSEE‐R were 0.5 mg/mL and 0.25 mg/mL, respectively, and there was no cytotoxic influence in the anti‐adhesion tests, as these were performed at much lower concentrations of these tested plant extracts. Adhesion of <italic>C. jejuni</italic> to pig (PSI) and human foetal (H4) small‐intestine cell lines was significantly decreased at lower concentrations (0.2 to 50 µg/mL). In the same concentration range, the invasiveness of <italic>C. jejuni</italic> in PSI cells was reduced by 45% to 65% when they were treated with SEE or hdSEE‐R. The hdSEE‐R represents a bioactive waste with a high phenolic content and an anti‐adhesive activity against <italic>C. jejuni</italic> and thus has the potential for use in pharmaceutical and food products. Copyright © 2015 John Wiley & Sons, Ltd.</p> </abstract>