Distinct serum proteome profiles associated with collagen‐induced arthritis and complete Freund's adjuvant‐induced inflammation in CD38−/− mice: The discriminative power of protein species or proteoforms. Issue 19 (28th August 2015)

• Record Type: Journal Article
• Authors: Rosal‐Vela, Antonio; García‐Rodríguez, Sonia; Postigo, Jorge; Iglesias, Marcos; Longobardo, Victoria; Lario, Antonio; Merino, Jesús; Merino, Ramón; Zubiaur, Mercedes; Sancho, Jaime
• Is Part Of: Proteomics. Volume 15:Issue 19(2015:Oct.)
• Page Start: 3382
• Page End: 3393
• Publication Date: 2015-08-28
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201400536
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Collagen‐type‐II‐induced arthritis (CIA) is an autoimmune disease, which involves a complex host systemic response including inflammatory and autoimmune reactions. CIA is milder in <italic>CD38<sup>−/−</sup></italic> than in wild‐type (WT) mice. ProteoMiner‐equalized serum samples were subjected to 2D‐DiGE and MS‐MALDI‐TOF/TOF analyses to identify proteins that changed in their relative abundances in <italic>CD38<sup>−/−</sup></italic> versus WT mice either with arthritis (CIA<sup>+</sup>), with no arthritis (CIA<sup>−</sup>), or with inflammation (complete Freund's adjuvant (CFA)‐treated mice). Multivariate analyses revealed that a multiprotein signature (<italic>n</italic> = 28) was able to discriminate CIA<sup>+</sup> from CIA<sup>−</sup> mice, and WT from <italic>CD38<sup>−/−</sup></italic> mice within each condition. Likewise, a distinct multiprotein signature (<italic>n</italic> = 16) was identified which differentiated CIA<sup>+</sup><italic>CD38<sup>−/−</sup></italic> mice from CIA<sup>+</sup> WT mice, and lastly, a third multiprotein signature (<italic>n</italic> = 18) indicated that CD38<sup>−/−</sup> and WT mice could be segregated in response to CFA treatment. Further analyses showed that the discriminative power to distinguish these groups was reached at protein species level and not at the protein level. Hence, the need to identify and quantify proteins at protein species level to better correlate proteome changes with disease processes. It is crucial for plasma proteomics at the low‐abundance protein species level to apply the ProteoMiner enrichment. All MS data have been deposited in the ProteomeXchange with identifiers PXD001788, PXD001799 and PXD002071 (<ext-link ext-link-type="uri" xlink:href="http://proteomecentral.proteomexchange.org/dataset/PXD001788" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">http://proteomecentral.proteomexchange.org/dataset/PXD001788</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://proteomecentral.proteomexchange.org/dataset/PXD001799" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">http://proteomecentral.proteomexchange.org/dataset/PXD001799</ext-link> and <ext-link ext-link-type="uri" xlink:href="http://proteomecentral.proteomexchange.org/dataset/PXD002071" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">http://proteomecentral.proteomexchange.org/dataset/PXD002071</ext-link>).</p> </abstract>