An Involvement of PI3‐K/Akt Activation and Inhibition of AIF Translocation in Neuroprotective Effects of Undecylenic Acid (UDA) Against Pro‐Apoptotic Factors‐Induced Cell Death in Human Neuroblastoma SH‐SY5Y Cells. Issue 12 (8th October 2015)

• Record Type: Journal Article
• Authors: Jantas, Danuta; Piotrowski, Marek; Lason, Wladyslaw
• Is Part Of: Journal of cellular biochemistry. Volume 116:Issue 12(2015:Dec.)
• Page Start: 2882
• Page End: 2895
• Publication Date: 2015-10-08
• Subjects: Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.25236
• Languages: English
• ISSNs: 0730-2312

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jcb25236-sec-0001" sec-type="section"> <p>Undecylenic acid (UDA), a naturally occurring 11‐carbon unsaturated fatty acid, has been used for several years as an economical antifungal agent and a nutritional supplement. Recently, the potential usefulness of UDA as a neuroprotective drug has been suggested based on the ability of this agent to inhibit μ‐calpain activity. In order to verify neuroprotective potential of UDA, we tested protective efficacy of this compound against cell damage evoked by pro‐apoptotic factors (staurosporine and doxorubicin) and oxidative stress (hydrogen peroxide) in human neuroblastoma SH‐SY5Y cells. We showed that UDA partially protected SH‐SY5Y cells against the staurosporine‐ and doxorubicin‐evoked cell death; however, this effect was not connected with its influence on caspase‐3 activity. UDA decreased the St‐induced changes in mitochondrial and cytosolic AIF level, whereas in Dox‐model it affected only the cytosolic AIF content. Moreover, UDA (1–40 μM) decreased the hydrogen peroxide‐induced cell damage which was connected with attenuation of hydrogen peroxide‐mediated necrotic (PI staining, ADP/ATP ratio) and apoptotic (mitochondrial membrane potential, caspase‐3 activation, AIF translocation) changes. Finally, we demonstrated that an inhibitor of PI3‐K/Akt (LY294002) but not MAPK/ERK1/2 (U0126) pathway blocked the protection mediated by UDA in all tested models of SH‐SY5Y cell injury. These in vitro data point to UDA as potentially effective neuroprotectant the utility of which should be further validated in animal studies. J. Cell. Biochem. 116: 2882–2895, 2015. © 2015 Wiley Periodicals, Inc.</p> </sec> </abstract>