Expression, correlation, and prognostic value of TRAF2 and TRAF4 expression in malignant plural effusion cells in human breast cancer. Issue 11 (31st August 2015)

• Record Type: Journal Article
• Authors: Zhao, Zhi‐Juan; Ren, Hua‐Yan; Yang, Fan; Wang, Jian; Wu, Guang‐Ping; Mi, Xiao‐Yi
• Is Part Of: Diagnostic cytopathology. Volume 43:Issue 11(2015:Nov.)
• Page Start: 897
• Page End: 903
• Publication Date: 2015-08-31
• Subjects: Cytodiagnosis -- Periodicals; Pathology, Cellular -- Periodicals; Cytology -- Periodicals; 616.07582
• DOI: 10.1002/dc.23330
• Languages: English
• ISSNs: 8755-1039

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="dc23330-sec-0001" sec-type="section"> <title>Background</title> <p>TRAF2 and TRAF4, members of the tumor necrosis factor receptor‐ associated factor family of intracellular signal transduction proteins, are associated with breast cancer progression and metastasis.</p> </sec> <sec id="dc23330-sec-0002" sec-type="section"> <title>Methods</title> <p>We collected malignant serous effusion cells from the patients with breast cancer (n = 46). Cell blocks prepared from plural effusions (n = 46) and primary breast cancer (n = 50), lymph node metastases (n = 50), and normal breast tissue specimens (n = 30). The immunohistochemistry was performed for the detection of TRAF2 and TRAF4 expression with the correlation of their expression with clinicopathological parameters and survival rate analyzed.</p> </sec> <sec id="dc23330-sec-0003" sec-type="section"> <title>Results</title> <p>Compared with normal breast tissues, TRAF2 expression was upregulated, and nuclear TRAF4 expression was downregulated in malignant pleural effusion cells, primary tumors, and lymph node metastases (P < 0.05). Multivariate analysis revealed TRAF2 expression in pleural effusions was associated with the molecular/pathological type, venous invasion, and lymph node metastasis, while nuclear TRAF4 expression was associated with age, tumor size, venous invasion, and lymph node metastasis, clinical staging, molecular/pathological subtype and p53 status (P < 0.05). There was a significant positive correlation between TRAF2 and TRAF4 expression levels in malignant pleural effusion cells (r = 0.937; P < 0.01). Kaplan‐Meire analysis demonstrated a close correlation of TRAF2 and TRAF4 expression in malignant pleural effusion cells with cumulative overall survival (P < 0.05).</p> </sec> <sec id="dc23330-sec-0004" sec-type="section"> <title>Conclusion</title> <p>TRAF2 and nuclear TRAF4 expression in malignant pleural effusion cells may represent potential prognostic factors and biomarkers of invasion and metastasis in breast cancer. Diagn. Cytopathol. 2015;43:897–903. © 2015 Wiley Periodicals, Inc.</p> </sec> </abstract>