High prevalence of the MYD88 mutation in testicular lymphoma: Immunohistochemical and genetic analyses. (4th August 2015)

• Record Type: Journal Article
• Authors: Oishi, Naoki; Kondo, Tetsuo; Nakazawa, Tadao; Mochizuki, Kunio; Tanioka, Fumihiko; Oyama, Toshio; Yamamoto, Tomoko; Iizuka, Junpei; Tanabe, Kazunari; Shibata, Noriyuki; Kirito, Keita; Katoh, Ryohei
• Is Part Of: Pathology international. Volume 65:Number 10(2015:Oct.)
• Page Start: 528
• Page End: 535
• Publication Date: 2015-08-04
• Subjects: Pathology -- Periodicals; 616.07
• DOI: 10.1111/pin.12336
• Languages: English
• ISSNs: 1320-5463

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The activating mutation of <italic>MYD88</italic> has been identified in diffuse large B‐cell lymphoma (DLBCL). We investigated the mutational status and both the gene amplification and protein expression of <italic>MYD88</italic> in 23 cases of testicular DLBCL. To detect the <italic>MYD88</italic> mutations, we employed the allele‐specific PCR and Sanger sequencing. <italic>MYD88</italic> gene amplification and protein expression were analyzed by quantitative PCR and by immunohistochemistry, respectively. There were 17 cases of primary testicular DLBCL: 94% (16/17) exhibited a non‐Germinal center B‐cell (non‐GCB) subtype, 82% (14/17) showed the <italic>MYD88</italic> L265P, and 65% (11/17) had intense expression of <italic>MYD88</italic>. When compared with normal lymph nodes, the <italic>MYD88</italic> is significantly amplified in primary testicular DLBCL. However, the amplification status showed no correlation with its mutational status or protein expression. Moreover, neither the <italic>MYD88</italic> mutational status nor the expression pattern affected overall survival. Six cases were secondary testicular DLBCL with an 83% (5/6) and an 80% (4/5) incidence of the non‐GCB subtype and of the <italic>MYD88</italic> L265P, respectively. In conclusion, we demonstrated a high prevalence of the non‐GCB subtype and the common <italic>MYD88</italic> L265P in both primary and secondary testicular DLBCL. Our data suggest that the <italic>MYD88</italic> mutation is a fairly consistent genetic feature in testicular DLBCL.</p> </abstract>