Molecular characterization and expression analysis of interferon-gamma in the large yellow croaker Larimichthys crocea. Issue 2 (October 2015)

• Record Type: Journal Article
• Authors: Chen, Ruan-Ni; Su, Yong-Quan; Wang, Jun; Liu, Min; Qiao, Ying; Mao, Yong; Ke, Qiao-Zhen; Han, Kun-Huang; Zheng, Wei-Qiang; Zhang, Jian-She; Wu, Chang-Wen
• Is Part Of: Fish & shellfish immunology. Volume 46:Issue 2(2015:Oct.)
• Page Start: 596
• Page End: 602
• Publication Date: 2015-10
• Subjects: Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.07.008
• Languages: English
• ISSNs: 1050-4648

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">The large yellow croaker <italic>Larimichthys crocea</italic> is an important mariculture fish species in China, and the bacterium <italic>Vibrio harveyi</italic> (<italic>V. harveyi</italic>) and the ciliate protozoan <italic>Cryptocaryon irritans</italic> (<italic>C. irritans</italic>) are the two major pathogens in its aquaculture sector. Interferon-gamma (IFN-γ) plays important roles in regulating both innate and cell mediated immune responses as an inflammatory cytokine. In this study, we obtained the nucleotide sequence of IFN-γ from the large yellow croaker (LcIFN-γ). The phylogenetic relationship tree of 18 available IFN-γ genes was constructed based on their sequences. Expression analyses in 10 various tissues were conducted after the croaker challenged with <italic>V. harveyi</italic> and <italic>C. irritans</italic>, respectively. Real time PCR analysis showed that the expression of LcIFN-γ was observed broadly in health individuals. After injected with <italic>V. harveyi</italic>, the 10 tissues had a higher expression of IFN-γ at the first day (1 d); only spleen, muscle, intestine, heart and skin had higher expressions after infected with <italic>C. irritans</italic> at 1 d. Major immune tissues (skin, gill, head kidney and spleen) and detoxification tissue (liver) were sampled at 0 h, 6 h, 1 d, 2 d, 3 d, 4 d, 5 d and 7 d to understand the expression trends of LcIFN-γ after challenged with <italic>C. irritans</italic>. The expressions of LcIFN-γ in skin and gill (the primary immune organs) showed a clear correlative relationship with the life cycle of <italic>C. irritans</italic>.</p> </sec> </abstract>