A new method for ABO genotyping using fluorescence melting curve analysis based on peptide nucleic acid probes. Issue 5 (September 2015)

• Record Type: Journal Article
• Authors: Lee, Kyungmyung; Park, Hyun-Chul; An, Sanghyun; Ahn, Eu-Ree; Lee, Yang-Han; Kim, Mi-Jung; Lee, Eun-jung; Park, Jae Sin; Jung, Jin Wook; Lim, Sikeun
• Is Part Of: Legal medicine. Volume 17:Issue 5(2015:Sep.)
• Page Start: 334
• Page End: 339
• Publication Date: 2015-09
• Subjects: Medical jurisprudence -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Medical jurisprudence; Periodicals; 614.1
• DOI: 10.1016/j.legalmed.2015.04.001
• Languages: English
• ISSNs: 1344-6223

Abstract

<abstract xml:lang="en" abstract-type="author-highlights" id="ab005"> <title> <x xml:space="preserve">Abstract</x> </title> <sec> <title id="st065">Highlights</title> <p id="sp0005"> <list id="l0005"> <list-item id="o0005"> <label></label> <p id="p0110">We developed PNA probe-based FMCA for ABO genotyping.</p> </list-item> <list-item id="o0010"> <label></label> <p id="p0115">This method allowed for simultaneous detection of three SNP sites in the <italic>ABO</italic> gene.</p> </list-item> <list-item id="o0015"> <label></label> <p id="p0120">Eighty samples were successfully genotyped.</p> </list-item> <list-item id="o0020"> <label></label> <p id="p0125">Reactions were performed in one single closed tube in less than 3 h.</p> </list-item> <list-item id="o0025"> <label></label> <p id="p0130">This method may be an effective and helpful tool in forensic investigations.</p> </list-item> </list> </p> </sec> </abstract>