A Set of Homo‐Oligomeric Standards Allows Accurate Protein Counting. (20th August 2015)

• Record Type: Journal Article
• Authors: Finan, Kieran; Raulf, Anika; Heilemann, Mike
• Is Part Of: Angewandte Chemie. Volume 127:Number 41(2015)
• Page Start: 12217
• Page End: 12220
• Publication Date: 2015-08-20
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201505664
• Languages: English
• ISSNs: 0044-8249

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Techniques based on fluorescence microscopy are increasingly used to count proteins in cells, but few stoichiometrically well‐defined standards are available to test their accuracy. A selection of bacterial homo‐oligomers were developed that contain 10–24 subunits and fully assemble when expressed in mammalian cells, and they can be used to easily validate/calibrate molecular counting methods. The utility of these standards was demonstrated by showing that nuclear pores contain 32 copies of the Nup107 complex.</p> </abstract>