Adipose tissue mitochondrial respiration and lipolysis before and after a weight loss by diet and RYGB. (4th September 2015)

• Record Type: Journal Article
• Authors: Hansen, Merethe; Lund, Michael T.; Gregers, Emilie; Kraunsøe, Regitze; Van Hall, Gerrit; Helge, Jørn W.; Dela, Flemming
• Is Part Of: Obesity. Volume 23:Number 10(2015:Oct.)
• Page Start: 2022
• Page End: 2029
• Publication Date: 2015-09-04
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.21223
• Languages: English
• ISSNs: 1930-7381

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="oby21223-sec-0001" sec-type="section"> <title>Objective</title> <p>To study adipose tissue mitochondrial respiration and lipolysis following a massive weight loss.</p> </sec> <sec id="oby21223-sec-0002" sec-type="section"> <title>Methods</title> <p>High resolution respirometry of adipose tissue biopsies and tracer determined whole body lipolysis. Sixteen obese patients with type 2 diabetes (T2DM) and 27 without (OB) were studied following a massive weight loss by diet and Roux‐en‐Y gastric bypass (RYGB).</p> </sec> <sec id="oby21223-sec-0003" sec-type="section"> <title>Results</title> <p>The mitochondrial respiratory rates were similar in OB and T2DM, and the mass‐specific oxygen flux increased significantly 4 and 18 months post‐surgery (<italic>P</italic> < 0.05). With normalization to mitochondrial content, no differences in oxidative capacity after RYGB were seen. The ratio between the oxidative phosphorylation system capacity (<italic>P</italic>) and the capacity of the electron transfer system (<italic>E</italic>) increased 18 months after RYGB in both groups (<italic>P</italic> < 0.05). Lipolysis per fat mass was similar in the two groups and was increased (<italic>P</italic> < 0.05) and lipid oxidation during hyperinsulinemia decreased 4 months post‐surgery. In T2DM, visceral fat mass was always higher relative to the body fat mass (%) compared to OB.</p> </sec> <sec id="oby21223-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Adipose tissue mitochondrial respiratory capacity increases with RYGB. Adipocytes adapt to massive weight loss by increasing the phosphorylation system ratio (<italic>P/E</italic>), suggesting an increased ability to oxidize substrates after RYGB. Lipolysis increases in the short term post‐surgery, and insulin sensitivity for suppression of lipolysis increases with RYGB.</p> </sec> </abstract>