Controlled release of simvastatin‐loaded thermo‐sensitive PLGA‐PEG‐PLGA hydrogel for bone tissue regeneration: in vitro and in vivo characteristics. Issue 11 (29th May 2015)

• Record Type: Journal Article
• Authors: Yan, Qi; Xiao, Li‐Qun; Tan, Lei; Sun, Wei; Wu, Tao; Chen, Liang‐Wen; Mei, Yan; Shi, Bin
• Is Part Of: Journal of biomedical materials research. Volume 103:Issue 11(2015:Nov.)
• Page Start: 3580
• Page End: 3589
• Publication Date: 2015-05-29
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35499
• Languages: English
• ISSNs: 1549-3296

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Reports on the local delivery of drug loaded injectable hydrogels for bone regeneration are currently limited. This study assessed the effect of controlled simvastatin (SIM) release from a thermo‐sensitive hydrogel <italic>in vitro</italic> and <italic>in vivo</italic>. We successfully manufactured and evaluated thermo‐sensitive poly(<sc>d, l</sc>‐lactide‐<italic>co</italic>‐glycolide)‐poly(ethylene glycol)‐poly(<sc>d, l</sc>‐lactide‐<italic>co</italic>‐glycolide) triblock copolymers (PLGA‐PEG‐PLGA) loaded with SIM. The osteogenic effect of this hydrogel was tested <italic>in vitro</italic> and <italic>in vivo</italic>. MC‐3T3 E1 cells proliferation and osteoblastic differentiation was analyzed after cultivation with the hydrogel extracts. Cells co‐cultured with SIM/PLGA‐PEG‐PLGA extracts showed an increase in mineralization and osteogenic gene expression compared to the other two groups. Additionally, the characteristics of this composite <italic>in vivo</italic> were demonstrated using a rat bone defect model. The bone defects injected with SIM/PLGA‐PEG‐PLGA hydrogel showed increased new bone formation compared to samples treated with PLGA‐PEG‐PLGA and control samples. The results of this study suggest that SIM/PLGA‐PEG‐PLGA might provide potential therapeutic value for bone healing. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3580–3589, 2015.</p> </abstract>