Endoscopic delivery of enteric neural stem cells to treat Hirschsprung disease. Issue 10 (17th July 2015)

• Record Type: Journal Article
• Authors: Cheng, L. S.; Hotta, R.; Graham, H. K.; Nagy, N.; Goldstein, A. M.; Belkind‐Gerson, J.
• Is Part Of: Neurogastroenterology & motility. Volume 27:Issue 10(2015:Oct.)
• Page Start: 1509
• Page End: 1514
• Publication Date: 2015-07-17
• Subjects: Gastrointestinal system -- Motility -- Periodicals; Gastrointestinal system -- Innervation -- Periodicals; 616.33
• DOI: 10.1111/nmo.12635
• Languages: English
• ISSNs: 1350-1925

Abstract

<abstract abstract-type="main" id="nmo12635-abs-0001"> <title>Abstract</title> <sec id="nmo12635-sec-0001" sec-type="section"> <title>Background</title> <p>Transplantation of enteric neural stem cells (ENSC) holds promise as a potential therapy for enteric neuropathies, including Hirschsprung disease. Delivery of transplantable cells via laparotomy has been described, but we propose a novel, minimally invasive endoscopic method of cell delivery.</p> </sec> <sec id="nmo12635-sec-0002" sec-type="section"> <title>Methods</title> <p>Enteric neural stem cells for transplantation were cultured from dissociated gut of postnatal donor mice. Twelve recipient mice, including Ednrb<sup>−/−</sup> mice with distal colonic aganglionosis, underwent colonoscopic injection of ENSC under direct vision using a 30‐gauge Hamilton needle passed through a rigid cystoureteroscope. Cell engraftment, survival, and neuroglial differentiation were studied 1–4 weeks after the procedure.</p> </sec> <sec id="nmo12635-sec-0003" sec-type="section"> <title>Key Results</title> <p>All recipient mice tolerated the procedure without complications and survived to sacrifice. Transplanted cells were found within the colonic wall in 9 of 12 recipient mice with differentiation into enteric neurons and glia.</p> </sec> <sec id="nmo12635-sec-0004" sec-type="section"> <title>Conclusions & Inferences</title> <p>Endoscopic injection of ENSC is a safe and reliable method for cell delivery, and can be used to deliver a large number of cells to a specific area of disease. This minimally invasive endoscopic approach may prove beneficial to future human applications of cell therapy for neurointestinal disease.</p> </sec> </abstract>