Farm exposures are associated with lower percentage of circulating myeloid dendritic cell subtype 2 at age 6. Issue 10 (21st July 2015)

• Record Type: Journal Article
• Authors: Martikainen, M.‐V.; Kääriö, H.; Karvonen, A.; Schröder, P. C.; Renz, H.; Kaulek, V.; Dalphin, J.‐C.; von Mutius, E.; Schaub, B.; Pekkanen, J.; Hirvonen, M.‐R.; Roponen, M.
• Is Part Of: Allergy. Volume 70:Issue 10(2015:Oct.)
• Page Start: 1278
• Page End: 1287
• Publication Date: 2015-07-21
• Subjects: Allergy -- Periodicals; 616.97
• DOI: 10.1111/all.12682
• Languages: English
• ISSNs: 0105-4538

Abstract

<abstract abstract-type="main" id="all12682-abs-0001"> <title>Abstract</title> <sec id="all12682-sec-0001" sec-type="section"> <title>Background</title> <p>Early life farm exposures have been shown to decrease the risk of allergic diseases. Dendritic cells (DCs) may mediate asthma‐protective effect of farm exposures as they play an important role in the development of immunity and tolerance. Our aim was to investigate whether the numbers and phenotypes of circulating DCs at age 6 are associated with farming, asthma, and atopy in a selected sample of French and Finnish children from the PASTURE study.</p> </sec> <sec id="all12682-sec-0002" sec-type="section"> <title>Methods</title> <p>We studied 82 farm and 86 nonfarm children with and without asthma. Using flow cytometry, BDCA1+ CD11c+ myeloid DC1s (mDC1), BDCA3+<sup>high</sup> mDC2s and BDCA2+ plasmacytoid DCs (pDCs) were identified and expressions of CD86, immunoglobulin‐like transcript 3 (ILT3) and ILT4 were analyzed. Questionnaires were used to assess prenatal and lifetime patterns of farm exposures and to define asthma. Atopic sensitization was defined by specific IgE measurements.</p> </sec> <sec id="all12682-sec-0003" sec-type="section"> <title>Results</title> <p>The percentage of mDC2 cells was lower in farm children (0.033 ± 0.001) than in nonfarm children (0.042 ± 0.001; <italic>P </italic>= 0.008). Similar associations were found between mDC2 percentage and prenatal (<italic>P</italic> = 0.02) and lifetime exposure to farm milk (<italic>P</italic> = 0.03) and stables (<italic>P</italic> = 0.003), but these associations were not independent from farming. Asthma was positively associated with ILT4 + mDCs (<italic>P</italic> = 0.04) and negatively with CD86 + pDCs (<italic>P</italic> = 0.048) but only in nonfarm children.</p> </sec> <sec id="all12682-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Inverse association between farm exposure and mDC2 percentage suggest that this DC subset may play a role in farm‐related immunoregulation.</p> </sec> </abstract>