Clinical value of chip-based digital-PCR platform for the detection of circulating DNA in metastatic colorectal cancer. Issue 10 (October 2015)

• Record Type: Journal Article
• Authors: Sefrioui, David; Sarafan-Vasseur, Nasrin; Beaussire, Ludivine; Baretti, Marina; Gangloff, Alice; Blanchard, France; Clatot, Florian; Sabourin, Jean-Christophe; Sesboüé, Richard; Frebourg, Thierry; Michel, Pierre; Di Fiore, Frédéric
• Is Part Of: Digestive and liver disease. Volume 47:Issue 10(2015)
• Page Start: 884
• Page End: 890
• Publication Date: 2015-10
• Subjects: Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; 616.33005
• DOI: 10.1016/j.dld.2015.05.023
• Languages: English
• ISSNs: 1590-8658

Abstract

<abstract abstract-type="author" id="abs0005"> <title id="sect0005">Abstract</title> <sec> <title id="sect0010">Background</title> <p id="spar0005">The detection of circulating DNA is considered a promising strategy in cancer patients. Digital PCR has emerged as a sensitive method able to quantify both circulating free and tumour DNA.</p> </sec> <sec> <title id="sect0015">Aim</title> <p id="spar0010">The aim of this study was to prospectively evaluate the clinical value of a chip-based digital PCR for the detection of circulating DNA.</p> </sec> <sec> <title id="sect0020">Methods</title> <p id="spar0015">Digital PCR was used in 34 metastatic colorectal cancer patients to detect and quantify circulating free and tumour DNA based on <italic>K-ras</italic> mutational status. Clinical outcomes were analyzed according to circulating DNA measurements.</p> </sec> <sec> <title id="sect0025">Results</title> <p id="spar0020">Digital PCR yielded a detection rate of 69% for circulating tumour DNA. The median concentrations of circulating free and tumour DNA were 20 and 6.8 ng/mL, respectively, with significant correlation between both biomarkers (<italic>p</italic> < 0.001). Median overall survival was 4.8 months in patients with high circulating free DNA (>75% quartile) versus not reached in patients with a low level (<25% quartile) (<italic>p</italic> = 0.029). Moreover, median overall survival was significantly decreased in patients with detectable circulating tumour DNA compared to those without (respectively 11.8 months versus not reached, <italic>p</italic> = 0.04).</p> </sec> <sec> <title id="sect0030">Conclusions</title> <p id="spar0025">Chip-based digital PCR is a simple and non-invasive method allowing the efficient detection of circulating DNA. Our results highlight that levels of these circulating markers may have a potential prognostic value.</p> </sec> </abstract>