Activity of several kinds of drugs against Neospora caninum. Issue 6 (December 2015)

• Record Type: Journal Article
• Authors: Qian, Weifeng; Wang, Hui; Shan, Dan; Li, Bo; Liu, Jing; Liu, Qun
• Is Part Of: Parasitology international. Volume 64:Issue 6(2015:Dec.)
• Page Start: 597
• Page End: 602
• Publication Date: 2015-12
• Subjects: Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2015.08.002
• Languages: English
• ISSNs: 1383-5769

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="st0005">Abstract</title> <sec> <p id="sp0005">Neosporosis caused by <italic>Neospora caninum</italic> is a serious disease in cattle and dogs worldwide. It is the major cause of abortion and neonatal mortality in cattle. In this study, we evaluated the anti-<italic>N. caninum</italic> activity of Chinese medicine extracts (curcumin, artemether), herbicides (atrazine, glyphosate), anticoccidiosis drugs (toltrazuril and ponazuril), cyclophosphamide, diminazene aceturate and praziquantel <italic>in vitro</italic> using parasite growth, replication and host cell invasion assays in human foreskin fibroblast cultures. Curcumin, artemether, atrazine, toltrazuril and ponazuril exhibited inhibitory activity with 50% growth inhibitory concentration (IC<sub>50</sub>) of 1.1 ± 0.4, 1.0 ± 0.05, 11.2 ± 2.7, 30.3 ± 2.0 and 33.3 ± 4.1 μg/ml, respectively, in the growth inhibition assay. They were also active against protozoa replication, but only curcumin was effective against host cell invasion. Glyphosate, cyclophosphamide, diminazene aceturate and praziquantel were ineffective. In an <italic>in vivo</italic> infection model, curcumin showed no activity against <italic>N. caninum</italic> infection. We showed that curcumin, artemether, atrazine, toltrazuril, and ponazuril exhibited anti-<italic>N. caninum</italic> activity <italic>in vitro</italic>, providing important information for further studies on anti-<italic>N. caninum</italic> drugs.</p> </sec> </abstract>