Evaluation of rapid cell division in non‐uniform cell cycles. (15th July 2015)

• Record Type: Journal Article
• Authors: Lee, Juyun; Jeon, Wonju; Chang, Man; Han, Myung‐Soo
• Is Part Of: Journal of basic microbiology. Volume 55:issue 10(2015:Oct.)
• Page Start: 1159
• Page End: 1167
• Publication Date: 2015-07-15
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201400732
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201400732-sec-0001" sec-type="section"> <p>To better understand the mechanisms of development of harmful algal blooms (HABs), accurate estimates of species‐specific <italic>in situ</italic> growth rates are needed. HABs are caused by rapid cell division by the causative microorganisms. To accurately estimate the <italic>in situ</italic> growth rates of harmful algae having non‐uniform and/or irregular cell cycles, we modified a standard equation based on the cell cycle, and calculated the <italic>in situ</italic> growth rate to describe the process of bloom development in nature. Sampling of a developing bloom of <italic>Heterosigma akashiwo</italic> in Pohang Bay, Korea, was conducted every 3 h from 15:00 on August 2 to 07:00 on August 4, 2006. The amount of <italic>H. akashiwo</italic> DNA was measured using flow cytometry following tyramide signal amplification‐fluorescence <italic>in</italic><italic>situ</italic> hybridization. On August 2, the percentage of G1 phase cells decreased from 15:00 to 19:00 then increased until 22:00; it then decreased until 07:00 on August 3, followed by an increase to 10:00. This indicates the ability of the cells in nature to undergo more than one round of division per day. During the following night two rounds of division did not occur. The <italic>i</italic><italic>n situ</italic> growth rates estimated using the modified equation ranged from 0.31 to 0.53 d<sup>−1</sup>. We conclude that the use of this equation enables more accurate estimates of bloom formation by rapidly dividing cells.</p> </sec> </abstract>