Genotypes of ITS region of rRNA in Microcystis (Cyanobacteria) populations in Erhai Lake (China) and their correlation with eutrophication level. (22nd June 2015)

• Record Type: Journal Article
• Authors: Song, Gaofei; Jiang, Yongguang; Yu, Gongliang; Li, Renhui
• Is Part Of: Journal of basic microbiology. Volume 55:issue 10(2015:Oct.)
• Page Start: 1203
• Page End: 1211
• Publication Date: 2015-06-22
• Subjects: Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201500080
• Languages: English
• ISSNs: 0233-111X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jobm201500080-sec-0001" sec-type="section"> <p>Previous studies on spatiotemporal changes of <italic>Microcystis</italic> genotypes have shown that the existence and succession of dominant genotypes always occur in eutrophicated freshwater bodies. However, few studies have focused on the correlation between genotype composition and eutrophication level. In the present study, clone libraries of the internal transcribed spacer (ITS) of <italic>rrn</italic> operon were sequenced from <italic>Microcystis</italic> populations in Erhai Lake, a subtropical plateau lake in the preliminary eutrophication stage. The genotype composition of the <italic>Microcystis</italic> populations was highly variable at spatiotemporal scales, and 473 ITS genotypes were identified from the 800 ITS sequences obtained. However, no significantly dominant ITS genotypes existed in the lake. Comparison of Erhai Lake with four major lakes in China, namely, Taihu, Chaohu, Gucheng, and Shijiu Lakes, showed that the <italic>Microcystis</italic> ITS genotypes and genetic diversity were negatively correlated with eutrophication level. Extensive comparison of the <italic>Microcystis</italic> ITS genotypes from waters worldwide revealed that 440 ITS genotypes were unique to Erhai Lake, and no obvious phylogenetic correlations can be detected among the dominant genotypes from different water bodies. The high genetic diversity of the <italic>Microcystis</italic> populations in Erhai Lake may have resulted from the effect of the early stage of eutrophication.</p> </sec> </abstract>