Prevention of post‐traumatic reinnervation with microtubule inhibitors. (6th July 2015)

• Record Type: Journal Article
• Authors: Park, Andrea M.; Dhanda Patil, Reena; Paniello, Randal C.
• Is Part Of: Laryngoscope. Volume 125:Number 10(2015:Oct.)
• Page Start: E333
• Page End: E337
• Publication Date: 2015-07-06
• Subjects: Otolaryngology -- Periodicals; 617.51005
• DOI: 10.1002/lary.25258
• Languages: English
• ISSNs: 0023-852X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="lary25258-sec-0001" sec-type="section"> <title>Objectives/Hypothesis</title> <p>Functional recovery after a recurrent laryngeal nerve or facial nerve injury may be impaired due to aberrant reinnervation. Previous work in a rat peripheral nerve injury model found vincristine to be a potent inhibitor of reinnervation, and it has since been used to effectively block neural regeneration in other animal models. However, vincristine's narrow therapeutic index may limit its utility; therefore, another microtubule inhibitor, paclitaxel, which has a higher therapeutic index, was tested.</p> </sec> <sec id="lary25258-sec-0002" sec-type="section"> <title>Study Design</title> <p>Animal (rat) study.</p> </sec> <sec id="lary25258-sec-0003" sec-type="section"> <title>Methods</title> <p>After controlled injury to the rat posterior tibial (PT) nerve, the gastrocnemius/soleus complex was injected with saline (control, n = 14), vincristine (n = 30), or paclitaxel (n = 20). Injections without a crush injury were performed using saline (n = 5) or paclitaxel (n = 9). The functional recovery (FR) of the PT nerve was assessed using walking track analysis.</p> </sec> <sec id="lary25258-sec-0004" sec-type="section"> <title>Results</title> <p>At 6 weeks, controls had already recovered to baseline (FR = 1.0), whereas the paclitaxel group had FR = 0.724 ± 0.064 and the vincristine group had FR = 0.709 ± 0.078. At 6 months, the paclitaxel rats had FR = 0.798 ± 0.167 and the vincristine rats had FR = 0.754 ± 0.240. These differences were significantly different from baseline, but the two agents were not different from each other. Paclitaxel did not affect the FR in the absence of a nerve injury.</p> </sec> <sec id="lary25258-sec-0005" sec-type="section"> <title>Conclusions</title> <p>Intramuscular paclitaxel and vincristine both significantly inhibit regeneration of the PT nerve after crush injury for at least 6 months. Potential clinical uses of inhibition of reinnervation are discussed.</p> </sec> <sec id="lary25258-sec-0006" sec-type="section"> <title>Level of Evidence</title> <p>NA <italic>Laryngoscope</italic>, 125:E333–E337, 2015</p> </sec> </abstract>