A Cell‐Penetrating Foldamer with a Bioreducible Linkage for Intracellular Delivery of DNA. Issue 38 (5th August 2015)

• Record Type: Journal Article
• Authors: Douat, Céline; Aisenbrey, Christopher; Antunes, Stéphanie; Decossas, Marion; Lambert, Olivier; Bechinger, Burkhard; Kichler, Antoine; Guichard, Gilles
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 38(2015)
• Page Start: 11133
• Page End: 11137
• Publication Date: 2015-08-05
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201504884
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Despite significant advances in foldamer chemistry, tailored delivery systems based on foldamer architectures, which provide a high level of control over secondary structure, are curiously rare among non‐viral technologies for transporting nucleic acids into cells. A potent pH‐responsive, bioreducible cell‐penetrating foldamer (CPF) was developed through covalent dimerization of a short (8‐mer) amphipathic oligourea sequence bearing histidine‐type units. This CPF exhibits a high capacity to assemble with pDNA and mediates efficient delivery of nucleic acids into the cell. Furthermore, it does not adversely affect cellular viability and was shown to compare favorably with a cognate peptide transfection agent based on His‐rich sequences.</p> </abstract>