High-content screening imaging and real-time cellular impedance monitoring for the assessment of chemical's bio-activation with regards hepatotoxicity. Issue 7 (October 2015)

• Record Type: Journal Article
• Authors: Peyre, Ludovic; de Sousa, Georges; Barcellini-Couget, Sylvie; Luzy, Anne-Pascale; Zucchini-Pascal, Nathalie; Rahmani, Roger
• Is Part Of: Toxicology in vitro. Volume 29:Issue 7(2015)
• Page Start: 1916
• Page End: 1931
• Publication Date: 2015-10
• Subjects: Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2015.07.024
• Languages: English
• ISSNs: 0887-2333

Abstract

<abstract xml:lang="en" abstract-type="author-highlights" id="ab005"> <title id="st005">Highlights</title> <sec> <p id="sp0005"> <list id="l0005"> <list-item id="o0005"> <label></label> <p id="p0005">Neutral red uptake was the most robust assay used for the drug cytotoxicity evaluation on murine liver models.</p> </list-item> <list-item id="o0010"> <label></label> <p id="p0010">Real-time cellular impedance could detect bio-activation through revealing specific curve profiles and response kinetics.</p> </list-item> <list-item id="o0015"> <label></label> <p id="p0015">Real-time cellular impedance seems unsuitable for use in monitoring viability in primary cultures of rat hepatocytes.</p> </list-item> <list-item id="o0020"> <label></label> <p id="p0020">The contribution of HCS appears necessary to highlight disruptions to cellular function in response to drug exposure.</p> </list-item> <list-item id="o0025"> <label></label> <p id="p0025">Coupling neutral red assay, real-time cellular impedance and HCS represents a complementary strategy for drug assessment.</p> </list-item> </list> </p> </sec> </abstract>