Decreased level of cytotoxic T lymphocyte antigen-4 (CTLA-4) in patients with acute immune thrombocytopenia (ITP). Issue 4 (October 2015)

• Record Type: Journal Article
• Authors: Zhu, Feng; Qiao, Jianlin; Cao, Jiang; Sun, Hai-ying; Wu, Qing-yun; Sun, Zeng-tian; Zhao, Kai; Sang, Wei; Qi, Kun-ming; Zeng, Ling-yu; Li, Zhen-yu; Xu, Kai-lin
• Is Part Of: Thrombosis research. Volume 136:Issue 4(2015)
• Page Start: 797
• Page End: 802
• Publication Date: 2015-10
• Subjects: Thrombosis -- Periodicals; 616.135
• DOI: 10.1016/j.thromres.2015.07.017
• Languages: English
• ISSNs: 0049-3848

Abstract

<abstract abstract-type="author" id="ab0010"> <title id="st0045">Abstract</title> <sec> <title id="st0050">Introduction</title> <p id="sp0040">Previously, we demonstrated the importance of T-cell immune response cDNA 7 (TIRC7) in acute immune thrombocytopenia (ITP). As the downstream molecule of TIRC7, cytotoxic T lymphocyte antigen-4 (CTLA-4) has been verified its negative regulation of acute ITP. This study aimed to investigate the exact role of CTLA-4 and its relationship with TIRC7 in acute ITP.</p> </sec> <sec> <title id="st0055">Patients and methods</title> <p id="sp0045">37 patients with acute ITP were enrolled and received dexamethasone (40 mg/day) for 4 consecutive days. Patients who had platelet counts more than 50 × 10<sup>9</sup>/L or less were defined as responders or non-responders after treatment. The plasma, protein and mRNA levels of CTLA-4 and TIRC7 were monitored by ELISA, western blot and q-PCR, respectively.</p> </sec> <sec> <title id="st0060">Results</title> <p id="sp0050">After high-dose dexamethasone therapy, CTLA-4 levels were significantly elevated not only in acute ITP patients (P < 0.001; P < 0.0001) but also in acute ITP responders (P < 0.0001; P < 0.0001). The levels of CTLA-4 were negatively correlated with the levels of TIRC7 before and after treatment; IFN-γ (Th1), IL-17 (Th17) and IL-22 (Th22) levels were all elevated, which were decreased after treatment not only in patients with acute ITP (P < 0.01) but also in acute ITP responders (P < 0.01).</p> </sec> <sec> <title id="st0065">Conclusions</title> <p id="sp0055">CTLA-4 level might reflect treatment efficacy and it might be associated with the pathogenesis of acute ITP.</p> </sec> </abstract>