Tamoxifen persistently disrupts the humoral adaptive immune response of gilthead seabream (Sparus aurata L.). Issue 2 (December 2015)

• Record Type: Journal Article
• Authors: Rodenas, M.C.; Cabas, I.; Abellán, E.; Meseguer, J.; Mulero, V.; García-Ayala, A.
• Is Part Of: Developmental and comparative immunology. Volume 53:Issue 2(2015)
• Page Start: 283
• Page End: 292
• Publication Date: 2015-12
• Subjects: Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.06.014
• Languages: English
• ISSNs: 0145-305X

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">There is increasing concern about the possible effect of pharmaceutical compounds may have on the fish immune system. Bath exposition of 17α-ethynylestradiol (EE<sub>2</sub>), a synthetic estrogen used in oral contraceptives, altered the immune response of the gilthead seabream (<italic>Sparus aurata</italic> L.), a marine hermaphrodite teleost. Tamoxifen (Tmx) is a selective estrogen-receptor modulator used in hormone replacement therapy, the effects of which are unknown in fish immunity. This study aims to investigate the effects of dietary administration of EE<sub>2</sub> (5 μg/g food) and Tmx (100 μg/g food) on the immune response of gilthead seabream, and the capacity of the immune system to recover its functionality after a recovery period. The results show for the first time the reversibility of the effect of EE<sub>2</sub> and Tmx on the fish immune response. Tmx promoted a transient alteration in hepatic vitellogenin gene expression of a different magnitude to that produced by EE<sub>2</sub>. Both, EE<sub>2</sub> and Tmx inhibited the induction of interleukin-1β gene expression while reversed the inhibition of ROI production in leukocytes following vaccination. However, none of these effects were observed after ceasing EE<sub>2</sub> and Tmx exposure. EE<sub>2</sub> and Tmx stimulated the antibody response of vaccinated fish although Tmx, but not EE<sub>2</sub>, altered the antibody response and modulated the percentage of IgM<sup>+</sup> B lymphocytes of vaccinated fish during the recovery phase. Taken together, our results suggest that EE<sub>2</sub> and Tmx might alter the capacity of fish to appropriately respond to infection and show that Tmx has a long-lasting effect on humoral adaptive immunity.</p> </sec> </abstract>