ID: 16. Issue 1 (November 2015)

• Record Type: Journal Article
• Authors: Soufli, Imene; Toumi, Ryma; Rafa, Hayet; Amri, Manel; Touil-Boukoffa, Chafia
• Is Part Of: Cytokine. Volume 76:Issue 1(2015)
• Page Start: 66
• Publication Date: 2015-11
• Subjects: Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2015.08.046
• Languages: English
• ISSNs: 1043-4666

Abstract

<abstract xml:lang="en" abstract-type="author" id="ab005"> <title> <x xml:space="preserve">Abstract</x> </title> <sec> <p id="sp005">Inflammatory bowel disease is less common in countries where there is a greater risk of exposure to helminths. In our study, we examined the modulatory effect of the laminated layer extracted from the cyst wall of a helminth parasite, <italic>Echinococcus granulosus</italic>, on dextran sulfate sodium (DSS)-induced colitis in mice. Crude extract of <italic>E. granulosus</italic> laminated layer was injected intra-peritoneally daily, starting 3 days before colitis induction. The Disease Activity Index was monitored daily and histological scores were evaluated. Nitric oxide (NO) and cytokine levels (interferon <inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7mmq" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si1.gif" display="inline" overflow="scroll" id="d13e78" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">γ</mml:mi></mml:mrow></mml:math></alternatives></inline-formula> (IFN-<inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7s4k" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si2.gif" display="inline" overflow="scroll" id="d13e83" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">γ</mml:mi><mml:mo stretchy="false">)</mml:mo></mml:mrow></mml:math></alternatives></inline-formula>, tumor necrosis factor <inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7r88" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si3.gif" display="inline" overflow="scroll" id="d13e91" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">α</mml:mi></mml:mrow></mml:math></alternatives></inline-formula> (TNF-<inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7rgk" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si4.gif" display="inline" overflow="scroll" id="d13e96" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">α</mml:mi><mml:mo stretchy="false">)</mml:mo></mml:mrow></mml:math></alternatives></inline-formula> and interleukin 10 (IL-10)) were assessed. In addition, the colonic expression of inducible nitric oxide synthase (iNOS) and nuclear factor-<inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7k97" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si5.gif" display="inline" overflow="scroll" id="d13e103" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">κ</mml:mi></mml:mrow></mml:math></alternatives></inline-formula>B (NF-<inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7k97" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si5.gif" display="inline" overflow="scroll" id="d13e108" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">κ</mml:mi></mml:mrow></mml:math></alternatives></inline-formula>B) was examined. Importantly, our results indicate that hydatid laminated layer pretreatment significantly improved the clinical symptoms and histological scores observed during DSS-induced colitis. Furthermore, treatment with hydatid laminated layer caused a significant decrease in NO (<sup>∗∗</sup><bold><italic>p</italic></bold> < <bold>0.001</bold>), <bold>IFN</bold>-<bold>γ</bold> (<sup>∗</sup><italic>p</italic> < 0.01) and TNF-<inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7r88" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si3.gif" display="inline" overflow="scroll" id="d13e140" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">α</mml:mi></mml:mrow></mml:math></alternatives></inline-formula> production (<sup>∗</sup><italic>p</italic> < 0.05) and an increase in IL-10 production. These results were associated with localized down-regulation of iNOS and NF-<inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7k97" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si5.gif" display="inline" overflow="scroll" id="d13e153" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">κ</mml:mi></mml:mrow></mml:math></alternatives></inline-formula>B expression. Our results demonstrate the potent protective effect of hydatid laminated layer in DSS induced colitis through downregulation of IFN-<inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7mmq" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si1.gif" display="inline" overflow="scroll" id="d13e159" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">γ</mml:mi></mml:mrow></mml:math></alternatives></inline-formula> and TNF-<inline-formula><alternatives><inline-graphic xlink:href="ark:/27927/pgj2n6x7r88" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /><mml:math altimg="si3.gif" display="inline" overflow="scroll" id="d13e164" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:mi mathvariant="normal">α</mml:mi></mml:mrow></mml:math></alternatives></inline-formula> and up regulation IL-10 production. Furthermore, hydatid laminated layer played a beneficial role in maintaining the integrity of the intestinal mucosal barrier against DSS-induced injury suggesting the crucial role of helminth in immuno-protection against IBD.</p> </sec> </abstract>