Islet antigen-pulsed dendritic cells expressing ectopic IL-35Ig protect nonobese diabetic mice from autoimmune diabetes. Issue 2 (October 2015)

• Record Type: Journal Article
• Authors: Mondanelli, Giada; Volpi, Claudia; Bianchi, Roberta; Allegrucci, Massimo; Talesa, Vincenzo Nicola; Grohmann, Ursula; Belladonna, Maria Laura
• Is Part Of: Cytokine. Volume 75:Issue 2(2015)
• Page Start: 380
• Page End: 388
• Publication Date: 2015-10
• Subjects: Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2015.05.002
• Languages: English
• ISSNs: 1043-4666

Abstract

<abstract xml:lang="en" abstract-type="author-highlights" id="ab005"> <title id="st005">Highlights</title> <sec> <p id="sp0005"> <list id="l0005"> <list-item id="o0005"> <label></label> <p id="p0005">We built a murine single chain gene construct of IgG₃-tagged IL-35.</p> </list-item> <list-item id="o0010"> <label></label> <p id="p0010">Gene construct-transfected cells efficiently expressed the cytokine fusion protein.</p> </list-item> <list-item id="o0015"> <label></label> <p id="p0015">IL-35Ig-transfected dendritic cells (DC₃₅) were immunosuppressive in a skin test.</p> </list-item> <list-item id="o0020"> <label></label> <p id="p0020">IGRP-pulsed DC₃₅ protected prediabtic NOD mice from autoimmune diabetes.</p> </list-item> <list-item id="o0025"> <label></label> <p id="p0025">Protective effect of DC₃₅ correlated with the regulatory CD4⁺CD39⁺ T cell increase.</p> </list-item> </list> </p> </sec> </abstract>