Comparison between S+L− assay and LacZ marker rescue assay for detecting replication-competent gammaretroviruses. Issue 5 (September 2015)

• Record Type: Journal Article
• Authors: Hashimoto-Gotoh, A.; Yoshikawa, R.; Miyazawa, T.
• Is Part Of: Biologicals. Volume 43:Issue 5(2015:Sep.)
• Page Start: 363
• Page End: 368
• Publication Date: 2015-09
• Subjects: Biological products -- Standards -- Periodicals; Biological Products -- Periodicals; Biological Products -- standards -- Periodicals; Produits biologiques -- Normes -- Périodiques; Biological products -- Standards; Periodicals; 615.37
• DOI: 10.1016/j.biologicals.2015.06.007
• Languages: English
• ISSNs: 1045-1056

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">To avoid contamination of adventitious gammaretroviruses in biological products such as vaccines, it is necessary to check the master seed cells for manufacturing. There are several assays to detect infectious gammaretroviruses. Among these, sarcoma-positive, leukemia-negative (S+L−) assay is a classical infectivity assay, which is often recommended in governmental guidelines. The S+L− cells used in S+L− assay generate unique focus upon the infection of replication-competent gammaretroviruses. Although S+L− assay is well recognized for the detection, their applicability is questionable in some cases. On the other hand, LacZ marker rescue (LMR) assay detects infectious gammaretroviruses by transducing LacZ marker gene to the target cells, which shows <italic>lacZ</italic>-positive foci if the infectious virus is present. In this study, we compared LMR and S+L− assays for detection of a variety of endogenous and exogenous gammaretroviruses. As results, LMR assay could detect all gammaretroviruses examined. On the other hand, S+L− assay using feline S+L− cells, termed QN10S, could not detect porcine endogenous retrovirus (PERV) subgroups A/B. Further, S+L− mink cells could not detect feline leukemia virus subgroups B in addition to PERV-A/B. These data indicate that LMR assay is better suited to detect wider range of gammaretroviruses.</p> </sec> </abstract>