Correlation of tumor characteristics derived from DCE‐MRI and DW‐MRI with histology in murine models of breast cancer. (30th August 2015)

• Record Type: Journal Article
• Authors: Barnes, Stephanie L.; Sorace, Anna G.; Loveless, Mary E.; Whisenant, Jennifer G.; Yankeelov, Thomas E.
• Is Part Of: NMR in biomedicine. Volume 28:Number 10(2015:Oct.)
• Page Start: 1345
• Page End: 1356
• Publication Date: 2015-08-30
• Subjects: Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.3377
• Languages: English
• ISSNs: 0952-3480

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The purpose of this work was to determine the relationship between the apparent diffusion coefficient (ADC, from diffusion‐weighted (DW) MRI), the extravascular, extracellular volume fraction (<italic>v</italic><sub>e</sub>, from dynamic contrast‐enhanced (DCE) MRI), and histological measurement of the extracellular space fraction.</p> <p>Athymic nude mice were injected with either human epidermal growth factor receptor 2 positive (HER2+) BT474 (<italic>n</italic> = 15) or triple negative MDA‐MB‐231 (<italic>n</italic> = 20) breast cancer cells, treated with either Herceptin (<italic>n</italic> = 8), Abraxane (low dose <italic>n</italic> = 7, high dose <italic>n</italic> = 6), or saline (<italic>n</italic> = 7 for each cell line), and imaged using DW‐ and DCE‐MRI before, during, and after treatment. After the final imaging acquisition, the tissue was resected and evaluated by histological analysis. H&E‐stained central slices were scanned using a digital brightfield microscope and evaluated with thresholding techniques to calculate the extracellular space.</p> <p>For both BT474 and MDA‐MB‐231, the median ADC of the central slice exhibited a significantly positive correlation with the corresponding central slice extracellular space as measured by H&E (<italic>p</italic> = 0.03, <italic>p</italic> < 0.01, respectively). Median <italic>v</italic><sub>e</sub> calculated from the central slice showed differing results between the two cell lines. For BT474, a significant correlation between <italic>v</italic><sub>e</sub> and extracellular space was calculated (<italic>p</italic> = 0.02), while MDA‐MB‐231 tumors did not demonstrate a significant correlation (<italic>p</italic> = 0.64). Additionally, there was no correlation discovered between ADC and <italic>v</italic><sub>e</sub> with either whole tumor analysis or central slice analysis (<italic>p</italic> > 0.05).</p> <p>While ADC correlates well with the histologically determined fraction of extracellular space, these data add to the growing body of literature that suggests that <italic>v</italic><sub>e</sub> derived from DCE‐MRI is not a reliable biomarker of extracellular space for a range of physiological conditions. Copyright © 2015 John Wiley & Sons, Ltd.</p> </abstract>