Identification of a novel cyprinid herpesvirus 3 genotype detected in koi from the East Asian and South‐East Asian Regions. Issue 10 (9th October 2014)

• Record Type: Journal Article
• Authors: Chen, J; Chee, D; Wang, Y; Lim, G Y; Chong, S M; Lin, Y N; Huangfu, T
• Is Part Of: Journal of fish diseases. Volume 38:Issue 10(2015:Oct.)
• Page Start: 915
• Page End: 923
• Publication Date: 2014-10-09
• Subjects: Fishes -- Diseases -- Periodicals; 639.3
• DOI: 10.1111/jfd.12305
• Languages: English
• ISSNs: 0140-7775

Abstract

<abstract abstract-type="main" id="jfd12305-abs-0001"> <title>Abstract</title> <p>Cyprinid herpesvirus 3 (CyHV‐3) is a highly contagious virus that causes significant morbidity and mortality in common carp <italic>Cyprinus carpio</italic> L. and considered to be one of the most important pathogens of koi and common carp worldwide. Cyprinid herpesvirus 3 infected consignments imported from East Asian and South‐East Asian regions were identified during quarantine period in Singapore, and virus from a 2005 consignment was successfully isolated in koi fin cells. A combination of sequence analyses and duplex PCR were used to characterize 15 CyHV‐3 isolates detected in koi consignments between 2005 and 2011. Sequence analyses of the enlarged 9/5, SphI‐5 and TK gene regions identified both the Asian 1 (<italic>n</italic> = 11) and European 4 (<italic>n</italic> = 4) genotypes. Duplex PCR analysis of two variable marker regions between ORF29 and ORF30 (marker I) as well as ORF133 and its upstream region (marker II) revealed viruses of genotypes J (I<sup>++</sup>II<sup>+</sup>), U/I (I<sup>−−</sup>II<sup>−</sup>), an intermediate genotype (I<sup>++</sup>II<sup>−</sup>) and a novel genotype, I<sup>++</sup>II<sup>+Δ</sup>, which was identified in viruses from seven different consignments. This novel genotype has a 13‐bp deletion in marker II, while maintaining the I<sup>++</sup> allele of marker I. The I<sup>++</sup>II<sup>+Δ</sup> genotype may have emerged from East Asian and South‐East Asian regions in recent years.</p> </abstract>