Development and validation of a high performance liquid chromatography method for determination of 6‐benzyl‐1‐benzyloxymethyl‐5‐iodouracil (W‐1), a novel non‐nucleoside reverse transcriptase inhibitor and its application to a pharmacokinetic study in rats. (24th March 2015)

• Record Type: Journal Article
• Authors: Lu, Ying‐Yuan; Wang, Xin; Wang, Xiao‐Wei; Liu, Jun‐Yi; Li, Pu; Ren, Hong; Lou, Ya‐Qing; Lu, Chuang; Zhang, Guo‐Liang
• Is Part Of: Biomedical chromatography. Volume 29:Number 10(2015:Oct.)
• Page Start: 1548
• Page End: 1552
• Publication Date: 2015-03-24
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3457
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>A sensitive and selective high‐performance liquid chromatographic (HPLC) method for determination of 6‐benzyl‐1‐benzyloxymethyl‐5‐iodouracil (W‐1), a novel non‐nucleoside reverse transcriptase inhibitor in rat plasma, was developed and validated. Chromatographic separation of W‐1 and megestrol acetate (internal standard) was achieved on a reversed‐phase C₁₈ column at 25°C. The mobile phase was consisted of acetonitrile–water (60:40, v/v) and pumped at a flow rate of 1.0 mL/min. The ultraviolet (UV) detector was set at the absorption wavelength of 284 nm. The calibration curve for W‐1 was linear over the concentration range of 0.01–8 µg/mL and the lower limit of quantification was 10 ng/mL. The intra‐ and inter‐day precision and accuracy were &lt;8.9 and 5.3%, respectively. The extraction recoveries ranged from 97.9 to 101.6%. The validated HPLC method was successfully applied to a pharmacokinetic study of W‐1 in rats. Copyright © 2015 John Wiley &amp; Sons, Ltd.</p> </abstract>