Development and application of an analytical method for curdione quantification in pregnant sprague–dawley rats by LC‐MS/MS. (4th March 2015)

• Record Type: Journal Article
• Authors: Meng, Xiang; Zhang, Ting; Li, Ying; Pan, Qi; Jiang, Juan; Luo, Yongwei; Chong, Liming; Yang, Yang; Xu, Sichong; Zhou, Li; Sun, Zuyue
• Is Part Of: Biomedical chromatography. Volume 29:Number 10(2015:Oct.)
• Page Start: 1499
• Page End: 1505
• Publication Date: 2015-03-04
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3449
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The vaginal administration route suffers from relatively low absorption efficiency, which may hinder the identification of the toxicokinetics of curdione in pregnant women. A sensitive analytical method for determining the plasma concentration of curdione was developed and applied in the determination of curdione in pregnant Sprague–Dawley rats as a simulated model. Glimepiride was used as an internal standard and chromatographic separation was achieved on a Capcell Pak C<sub>18</sub> MGIII column. A gradient elution profile with 0.5% formic acid (A)–0.5% formic acid–acetonitrile (B) was selected as mobile phase. The selected reaction monitoring mode was used for quantification based on the target fragment ions <italic>m</italic>/<italic>z</italic> 237.2 to <italic>m</italic>/<italic>z</italic> 135.1 for curdione and <italic>m</italic>/<italic>z</italic> 491.3 to <italic>m</italic>/<italic>z</italic> 352.1 for the glimepiride. The standard curve was linear over the range of 0.5–500 ng/mL for curdione in rat plasma and yielded a consistent peak pattern, even at the lower limit of quantitation of 0.5 ng/mL. The retention times of curdione and IS were 6.55 and 6.59 min, respectively. The mean recovery of curdione in rat plasma was 95.5–101.1%. The intra‐day and inter‐day precisions were between 2.35 and 9.08%. This LC‐MS/MS method provides a simple and sensitive means for determining the plasma concentration. Copyright © 2015 John Wiley & Sons, Ltd.</p> </abstract>