Determination of corilagin in rat plasma using a liquid chromatography–electrospray ionization tandem mass spectrometric method. (24th March 2015)

• Record Type: Journal Article
• Authors: Chen, Qian Qian; Guo, Jianru; Fan, Hongyan; Wang, Caiyun; Xu, Fengguo; Zhang, Wei
• Is Part Of: Biomedical chromatography. Volume 29:Number 10(2015:Oct.)
• Page Start: 1553
• Page End: 1558
• Publication Date: 2015-03-24
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3458
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>A sensitive and simple liquid chromatography–tandem mass spectrometric (HPLC‐MS/MS) method for the determination of corilagin in rat plasma has been developed. Samples were prepared with protein precipitation method and analyzed with a triple quadrupole tandem mass spectrometer. We employed negative electrospray ionization as the ionization source and the analytes were detected in multiple reaction monitoring mode. Separation was achieved on a C₈ column eluted with mobile phase consisting of methanol–0.1% formic acid in a gradient mode at the flow rate of 0.3 mL/min. The total run time was 7.0 min.This method was proved to have good linearity in the concentration range of 2.5–1000.0 ng/mL. The lower limit of quantification of corilagin was 2.5 ng/mL. The intra‐ and inter‐day relative standard deviationa across three validation runs for four concentration levels were both &lt;9.8%. The relative error was within ±6.0%. This assay offers advantages in terms of expediency and suitability for the analysis of corilagin in rat plasma. The practical utility of this new HPLC‐MS/MS method was confirmed in pilot plasma concentration studies in rats following oral administration. Copyright © 2015 John Wiley &amp; Sons, Ltd.</p> </abstract>