Condensation Agents Determine the Temperature–Pressure Stability of F‐Actin Bundles. (5th August 2015)

• Record Type: Journal Article
• Authors: Gao, Mimi; Berghaus, Melanie; von der Ecken, Julian; Raunser, Stefan; Winter, Roland
• Is Part Of: Angewandte Chemie. Volume 127:Number 38(2015)
• Page Start: 11240
• Page End: 11244
• Publication Date: 2015-08-05
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201504247
• Languages: English
• ISSNs: 0044-8249

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Biological cells provide a large variety of rodlike filaments, including filamentous actin (F‐actin), which can form meshworks and bundles. One key question remaining in the characterization of such network structures revolves around the temperature and pressure stabilities of these architectures as a way to understand why cells actively use proteins for forming them. The packing properties of F‐actin in fascin‐ and Mg²⁺‐induced bundles are compared, and significantly different pressure‐temperature stabilities are observed because of marked differences in their nature of interaction, solvation, and packing efficiency. Moreover, differences are observed in their morphologies and disintegration scenarios. The pressure‐induced dissociation of the actin bundles is reminiscent of a single unbinding transition as observed in other soft elastic manifolds.</p> </abstract>