Analysis of MTR and MTRR Polymorphisms for Neural Tube Defects Risk Association. Issue 35 (September 2015)

• Record Type: Journal Article
• Authors: Wang, Yongxin; Liu, Yuan; Ji, Wenyu; Qin, Hu; Wu, Hao; Xu, Danshu; Tukebai, Turtuohut; Wang, Zengliang; Elsie, Baronia-Locson.
• Is Part Of: Medicine. Volume 94:Issue 35(2015)
• Publication Date: 2015-09
• Subjects: Medicine -- Periodicals; Medicine -- Periodicals; Médecine -- Périodiques; Geneeskunde; Medicine; Periodicals; Periodicals; 610.5
• DOI: 10.1097/MD.0000000000001367
• Languages: English
• ISSNs: 0025-7974

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <sec> <title>Abstract</title> <p>Neural tube defects (NTDs) are the most common congenital defects of the central nervous system among neonates and the folate status during pregnancy was considered as the most important etiopathogenesis of NTDs. Besides, methionine synthase (MTR) gene and methionine synthase reductase (MTRR) gene were folate metabolism involved genes and had been investigated in several previous studies with inconsistent results. Hence, we aimed to explore the association of 4 selected single-nucleotide polymorphisms (SNPs) on MTRR/MTR gene and the susceptibility of NTDs in a Chinese population.</p> <p>Seven SNPs were selected from HapMap databases with Haploview 4.2 software. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to genotype the polymorphisms from blood samples of 165 NTDs patients and 280 healthy controls. The correlation between these SNPs and NTDs risk was tested by Student <italic>t</italic> test and Chi-square test by STATA 11.0 software. Furthermore, we performed a meta-analysis of relevant studies to investigate the association between the SNPs <italic>MTRR</italic> 66A>G and <italic>MTR</italic> 2756A>G and the susceptibility of NTDs.</p> <p>An increased risk of NTDs was verified to be significantly associated with <italic>MTRR</italic> 66A>G (G allele vs. A allele: OR = 1.36 (1.03–1.80), <italic>P</italic> = 0.028; GG + AG vs. AA: OR = 1.60 (1.05–2.43), <italic>P</italic> = 0.027) and <italic>MTR</italic> 2756A>G (G allele vs. A allele: OR = 1.45 (1.06–1.98), <italic>P</italic> = 0.021; GG + AG vs. AA: OR = 1.51 (1.02–2.23), <italic>P</italic> = 0.038) in our study. However, the other SNPs in our analysis showed no significant association with NTDs risk (all <italic>P</italic> > 0.05). Furthermore, the result of the meta-analysis supported the association between MTRR 66A>G and NTDs risk (G allele vs. A allele: OR = 1.32, 95% CI = 1.09–1.61, GG + GA vs. AA: OR = 1.49, 95% CI = 1.06–2.09, GG vs. AA: OR = 1.61, 95% CI = 1.04–2.49).</p> <p>Our study confirmed that the <italic>MTRR</italic> 66A>G and <italic>MTR</italic> 2756A>G were significantly associated with the increased NTDs risk in a Chinese population. The further meta-analysis enhance that <italic>MTRR</italic> 66A>G was connected with the susceptibility of NTDs widely. Further investigations based on more detailed stratification were recommended.</p> </sec> </abstract>