Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens. Issue 9 (1st September 2015)

• Record Type: Journal Article
• Authors: Ye, Fuzhou; Wang, Chao; Fu, Qinqin; Zhang, Lian‐hui; Gao, Yong‐gui
• Is Part Of: Acta crystallographica. Volume 71:Issue 9(2015:Sep.)
• Page Start: 1139
• Page End: 1145
• Publication Date: 2015-09-01
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X15012881
• Languages: English
• ISSNs: 2053-230X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Two proteins, SghA and SghR, which were recently identified and characterized as novel bacterial virulence factors regulating the infection of plant hosts by <italic>Agrobacterium</italic>, were cloned, overexpressed and purified with high yield. Both SghA and SghR form dimers in solution. The purified SghA and SghR were crystallized and the crystals diffracted to 1.9 and 2.1 Å resolution, respectively. Data were collected and processed, and the crystallographic parameters were within acceptable ranges. These results will help in the determination of their structures in order to uncover the molecular mechanism of how these two proteins together control the release of plant defence signals against agrobacteria during pathogen–host interaction.</p> </abstract>