A DN-mda5 transgenic zebrafish model demonstrates that Mda5 plays an important role in snakehead rhabdovirus resistance. Issue 2 (August 2015)

• Record Type: Journal Article
• Authors: Gabor, K.A.; Charette, J.R.; Pietraszewski, M.J.; Wingfield, D.J.; Shim, J.S.; Millard, P.J.; Kim, C.H.
• Is Part Of: Developmental and comparative immunology. Volume 51:Issue 2(2015)
• Page Start: 298
• Page End: 304
• Publication Date: 2015-08
• Subjects: Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.01.006
• Languages: English
• ISSNs: 0145-305X

Abstract

<abstract abstract-type="author-highlights" id="ab0010"> <title id="st0010">Highlights</title> <sec> <p id="sp0010"> <list id="ulist0010"> <list-item id="u0010"> <label></label> <p id="p0010">Overexpression of <italic>mda5</italic> induced zebrafish IFN promoter activity and conferred protection against SHRV infection <italic>in vitro</italic>.</p> </list-item> <list-item id="u0015"> <label></label> <p id="p0015">A dominant negative <italic>mda5</italic> transgenic zebrafish was generated to assess the <italic>in vivo</italic> function of mda5.</p> </list-item> <list-item id="u0020"> <label></label> <p id="p0020">DN-<italic>mda5</italic> zebrafish resistance to SHRV infection is significantly reduced and leads to higher viral burden and mortality.</p> </list-item> <list-item id="u0025"> <label></label> <p id="p0025">Recombinant <italic>mda5</italic> confers protection against SHRV infection in DN-<italic>mda5</italic> zebrafish.</p> </list-item> </list> </p> </sec> </abstract>