Proteomic profile of the plant‐pathogenic oomycete Phytophthora capsici in response to the fungicide pyrimorph. Issue 17 (8th June 2015)

• Record Type: Journal Article
• Authors: Pang, Zhili; Chen, Lei; Miao, Jianqiang; Wang, Zhiwen; Bulone, Vincent; Liu, Xili
• Is Part Of: Proteomics. Volume 15:Issue 17(2015:Sep.)
• Page Start: 2972
• Page End: 2982
• Publication Date: 2015-06-08
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201400502
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Pyrimorph is a novel fungicide from the carboxylic acid amide (CAA) family used to control plant‐pathogenic oomycetes such as <italic>Phytophthora capsici</italic>. The proteomic response of <italic>P. capsici</italic> to pyrimorph was investigated using the iTRAQ technology to determine the target site of the fungicide and potential biomarker candidates of drug efficacy. A total of 1336 unique proteins were identified from the mycelium of wild‐type <italic>P. capsici</italic> isolate (Hd3) and two pyrimorph‐resistant mutants (R3‐1 and R3‐2) grown in the presence or absence of pyrimorph. Comparative analysis revealed that the three <italic>P. capsici</italic> isolates Hd3, R3‐1, and R3‐2 produced 163, 77, and 13 unique proteins, respectively, which exhibited altered levels of abundance in response to the pyrimorph treatment. Further investigations, using Cluster of Orthologous Groups of Proteins (COG) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified 35 proteins related to the mode of action of pyrimorph against <italic>P. capsici</italic> and 62 proteins involved in the stress response of <italic>P. capsici</italic> to pyrimorph. Many of the proteins with altered expression were associated with glucose and energy metabolism. Biochemical analysis using <sc>d</sc>‐[U‐<sup>14</sup>C]glucose verified the proteomics data, suggesting that the major mode of action of pyrimorph in <italic>P. capsici</italic> is the inhibition of cell wall biosynthesis. These results also illustrate that proteomics approaches are useful tools for determining the pathways targeted by novel fungicides as well as for evaluating the tolerance of plant pathogens to environmental challenges, such as the presence of fungicides.</p> </abstract>