Skin vaccination with live virus vectored microneedle arrays induce long lived CD8+ T cell memory. Issue 37 (8th September 2015)

• Record Type: Journal Article
• Authors: Becker, Pablo D.; Hervouet, Catherine; Mason, Gavin M.; Kwon, Sung-Yun; Klavinskis, Linda S.
• Is Part Of: Vaccine. Volume 33:Issue 37(2015)
• Page Start: 4691
• Page End: 4698
• Publication Date: 2015-09-08
• Subjects: Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2015.04.046
• Languages: English
• ISSNs: 0264-410X

Abstract

<abstract abstract-type="author" id="abs0005"> <title id="sect0005">Abstract</title> <sec> <p id="spar0005">A simple dissolvable microneedle array (MA) platform has emerged as a promising technology for vaccine delivery, due to needle-free injection with a formulation that preserves the immunogenicity of live viral vectored vaccines dried in the MA matrix. While recent studies have focused largely on design parameters optimized to induce primary CD8<sup>+</sup> T cell responses, the hallmark of a vaccine is synonymous with engendering long-lasting memory. Here, we address the capacity of dried MA vaccination to programme phenotypic markers indicative of effector/memory CD8<sup>+</sup> T cell subsets and also responsiveness to recall antigen benchmarked against conventional intradermal (ID) injection. We show that despite a slightly lower frequency of dividing T cell receptor transgenic CD8<sup>+</sup> T cells in secondary lymphoid tissue at an early time point, the absolute number of CD8<sup>+</sup> T cells expressing an effector memory (CD62L<sup>−</sup>CD127<sup>+</sup>) and central memory (CD62L<sup>+</sup>CD127<sup>+</sup>) phenotype during peak expansion were comparable after MA and ID vaccination with a recombinant human adenovirus type 5 vector (AdHu5) encoding HIV-1 gag. Similarly, both vaccination routes generated CD8<sup>+</sup> memory T cell subsets detected in draining LNs for at least two years post-vaccination capable of responding to secondary antigen. These data suggest that CD8<sup>+</sup> T cell effector/memory generation and long-term memory is largely unaffected by physical differences in vaccine delivery to the skin via dried MA or ID suspension.</p> </sec> </abstract>