Oakleaf: an S locus‐linked mutation of Primula vulgaris that affects leaf and flower development. Issue 1 (9th April 2015)

• Record Type: Journal Article
• Authors: Cocker, Jonathan M.; Webster, Margaret A.; Li, Jinhong; Wright, Jonathan; Kaithakottil, Gemy; Swarbreck, David; Gilmartin, Philip M.
• Is Part Of: New phytologist. Volume 208:Issue 1(2015:Oct. 01)
• Page Start: 149
• Page End: 161
• Publication Date: 2015-04-09
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.13370
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph13370-abs-0001"> <title>Summary</title> <p> <list id="nph13370-list-0001" list-type="bullet"> <list-item> <p>In <italic>Primula vulgaris</italic> outcrossing is promoted through reciprocal herkogamy with insect‐mediated cross‐pollination between pin and thrum form flowers. Development of heteromorphic flowers is coordinated by genes at the <italic>S</italic> locus. To underpin construction of a genetic map facilitating isolation of these <italic>S</italic> locus genes, we have characterised <italic>Oakleaf</italic>, a novel <italic>S</italic> locus‐linked mutant phenotype.</p> </list-item> <list-item> <p>We combine phenotypic observation of flower and leaf development, with classical genetic analysis and next‐generation sequencing to address the molecular basis of <italic>Oakleaf</italic>.</p> </list-item> <list-item> <p> <italic>Oakleaf</italic> is a dominant mutation that affects both leaf and flower development; plants produce distinctive lobed leaves, with occasional ectopic meristems on the veins. This phenotype is reminiscent of overexpression of Class I <italic>KNOX‐</italic>homeodomain transcription factors. We describe the structure and expression of all eight <italic>P. vulgaris </italic><italic>PvKNOX</italic> genes in both wild‐type and <italic>Oakleaf</italic> plants, and present comparative transcriptome analysis of leaves and flowers from <italic>Oakleaf</italic> and wild‐type plants.</p> </list-item> <list-item> <p> <italic>Oakleaf</italic> provides a new phenotypic marker for genetic analysis of the <italic>Primula S</italic> locus. We show that none of the Class I <italic>PvKNOX</italic> genes are strongly upregulated in <italic>Oakleaf</italic> leaves and flowers, and identify cohorts of 507 upregulated and 314 downregulated genes in the <italic>Oakleaf</italic> mutant.</p> </list-item> </list> </p> </abstract>