Preparation of pure, high titer, pseudoinfectious Flavivirus particles by hollow fiber tangential flow filtration and anion exchange chromatography. Issue 35 (20th August 2015)

• Record Type: Journal Article
• Authors: Mundle, Sophia T.; Giel-Moloney, Maryann; Kleanthous, Harry; Pugachev, Konstantin V.; Anderson, Stephen F.
• Is Part Of: Vaccine. Volume 33:Issue 35(2015)
• Page Start: 4255
• Page End: 4260
• Publication Date: 2015-08-20
• Subjects: Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2014.09.074
• Languages: English
• ISSNs: 0264-410X

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0005"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">A purification procedure for a replication-deficient flavivirus is described.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">Purification is achieved by tangential flow filtration followed by anion exchange chromatography.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">The use of a convective monolithic anion exchanger supports recovery of infectious virus.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">Purification results in ∼60% recovery of infectious virus titer.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">Vaccine virus with titers as high as 1 × 10⁹ FFU/mL have been prepared.</p> </list-item> </list> </p> </sec> </abstract>