Increasing Vero viable cell densities for yellow fever virus production in stirred-tank bioreactors using serum-free medium. Issue 35 (20th August 2015)

• Record Type: Journal Article
• Authors: Mattos, Diogo A.; Silva, Marlon V.; Gaspar, Luciane P.; Castilho, Leda R.
• Is Part Of: Vaccine. Volume 33:Issue 35(2015)
• Page Start: 4288
• Page End: 4291
• Publication Date: 2015-08-20
• Subjects: Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2015.04.050
• Languages: English
• ISSNs: 0264-410X

Abstract

<abstract abstract-type="author" id="abs0005"> <title id="sect0005">Abstract</title> <sec> <p id="spar0005">In this work, changes in Vero cell cultivation methods have been employed in order to improve cell growth conditions to obtain higher viable cell densities and to increase viral titers. The propagation of the 17DD yellow fever virus (YFV) in Vero cells grown on Cytodex I microcarriers was evaluated in 3-L bioreactor vessels. Prior to the current changes, Vero cells were repeatedly displaying insufficient microcarrier colonization. A modified cultivation process with four changes has resulted in higher cell densities and higher virus titers than previously observed for 17DD YFV.</p> </sec> </abstract>