In vitro osteoclast formation and resorption of silicon‐substituted hydroxyapatite ceramics. Issue 10 (22nd April 2015)

• Record Type: Journal Article
• Authors: Friederichs, Robert J.; Brooks, Roger A.; Ueda, Masato; Best, Serena M.
• Is Part Of: Journal of biomedical materials research. Volume 103:Issue 10(2015:Oct.)
• Page Start: 3312
• Page End: 3322
• Publication Date: 2015-04-22
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35470
• Languages: English
• ISSNs: 1549-3296

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Materials that participate in bone remodeling at the implant/tissue interface represent a modern tissue engineering approach with the aim of balancing implant resorption and nascent tissue formation. Silicon‐substituted hydroxyapatite (SiHA) ceramics are capable of stimulating new bone formation, but little is known about their interaction with osteoclasts (OC). The effects of soluble silicate and SiHA on OCs were investigated in this study. Soluble silicate below 500 μ<italic>M</italic> did not stimulate cell metabolism at 4 days or alter resorption area at 7 days on calcium phosphate discs. On sintered ceramics, OC numbers were similar on HA, Si<sub>0.3</sub>HA (0.5 wt % Si) and Si<sub>0.5</sub>HA (1.2 wt % Si) after 21 days <italic>in vitro</italic>, but actin ring sealing zone morphology on SiHA resembled that commonly found on bone or on carbonate‐substituted hydroxyapatite (CHA). Smaller and thicker actin rings on SiHA as compared to HA were probably the result of altered surface chemistry and solubility differences. The more stable sealing zones and increased lattice solubility likely contributed to increased individual pit volumes observed on Si<sub>0.5</sub>HA. The delayed formation of OCs on Si<sub>0.5</sub>HA (lower numbers at day 14) excludes earlier differentiation as a possible mechanism of increased individual OC pit volumes at later times (day 21). Materials characterization of Si containing biomaterials remains paramount as the Si type and amounts can subsequently impact downstream OC behaviour in a complex manner. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3312–3322, 2015.</p> </abstract>