Differentiation of healthy and malignant tissue in colon cancer patients using optical spectroscopy: A tool for image‐guided surgery. Issue 7 (20th July 2015)

• Record Type: Journal Article
• Authors: Langhout, G.C.; Spliethoff, J.W.; Schmitz, S.J.; Aalbers, A.G.J.; van Velthuysen, M.‐L.F.; Hendriks, B.H.W.; Ruers, T.J.M.; Kuhlmann, K.F.D.
• Is Part Of: Lasers in surgery and medicine. Volume 47:Issue 7(2015)
• Page Start: 559
• Page End: 565
• Publication Date: 2015-07-20
• Subjects: Lasers in medicine -- Periodicals; Lasers in surgery -- Periodicals; 617
• DOI: 10.1002/lsm.22388
• Languages: English
• ISSNs: 0196-8092

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="lsm22388-sec-0001" sec-type="section"> <title>Background</title> <p>Surgery for colorectal cancer aims for complete tumor resection. Optical‐based techniques can identify tumor and surrounding tissue through the tissue specific optical properties, absorption and scattering, which are both influenced by the biochemical and morphological composition of the tissue.</p> </sec> <sec id="lsm22388-sec-0002" sec-type="section"> <title>Objective</title> <p>To evaluate the feasibility of dual‐modality Diffuse Reflectance Spectroscopy–Fluorescence Spectroscopy (DRS‐FS) for discrimination between healthy and malignant tissue in colorectal surgery.</p> </sec> <sec id="lsm22388-sec-0003" sec-type="section"> <title>Methods</title> <p>Surgical specimens from colorectal cancer patients were measured immediately after resection using a fiber‐optic needle capable of dual‐modality DRS‐FS. Model‐based analyses were used to derive scattering and absorption coefficients and intrinsic fluorescence. Volume fractions of chromophores were estimated. Furthermore, optical data were recorded along a trajectory from healthy tissue towards tumor.</p> </sec> <sec id="lsm22388-sec-0004" sec-type="section"> <title>Results</title> <p>Spectral characteristics were identified in 1, 273 measured spectra from 21 specimens. Combined DRS and FS discriminated tumor from surrounding tissue with a sensitivity of 95% and a specificity of 88%. Significant spectral changes were seen along the trajectory from healthy tissue to tumor.</p> </sec> <sec id="lsm22388-sec-0005" sec-type="section"> <title>Conclusion</title> <p>This study demonstrates that dual‐modality DRS‐FS can identify colorectal cancer from surrounding healthy tissue. The quantification of comprehensible parameters allows robust classification and facilitates extrapolation towards the clinical setting. The technique, here demonstrated in a needle like probe, can be incorporated into surgical tools for optically guided surgery in the near future. Lasers Surg. Med. 47:559–565, 2015. © 2015 Wiley Periodicals, Inc.</p> </sec> </abstract>