TNF-308 G/A polymorphism and risk of systemic lupus erythematosus in the Polish population. Issue 5 (September 2015)

• Record Type: Journal Article
• Authors: Piotrowski, Piotr; Wudarski, Mariusz; Sowińska, Anna; Olesińska, Marzena; Jagodziński, Paweł P.
• Is Part Of: Modern rheumatology. Volume 25:Issue 5(2015)
• Page Start: 719
• Page End: 723
• Publication Date: 2015-09
• Subjects: Rheumatology -- Periodicals; 616.723005
• Languages: English
• ISSNs: 1439-7595

Abstract

<abstract> <title>Abstract</title> <p> <italic>Objectives.</italic> Numerous studies have been performed with <italic>TNF</italic>-α-308 G/A (rs1800629) single nuclear polymorphism (SNP) to evaluate the risk of SLE in various ethnicities. However, the significance of <italic>TNF-α</italic>-308 G/A in both clinical and laboratory studies of the disease remains unclear.</p> <p> <italic>Methods.</italic> Using a high-resolution melting curve analysis, we assessed the prevalence of <italic>TNF-α</italic>-308 G/A SNP in SLE patients (<italic>n</italic> = 262) and controls (<italic>n</italic> = 528) in a Polish population. We also assessed the contribution of this SNP to various clinical symptoms and the presence of autoantibodies in SLE patients.</p> <p> <italic>Results.</italic> The p-value obtained using a χ<sup>2</sup> test for the trend of <italic>TNF-α</italic>-308 G/A was statistically significant (<italic>p</italic><sub>trend</sub> = 0.0297). However, using logistic regression analysis for the presence of the HLA-DRB1*03:01 haplotype, we observed that the <italic>TNF-α</italic>-308 G/A SNP may be the DRB1*03:01-dependent risk factor of SLE in the Polish population. There was a significant contribution of <italic>TNF-α</italic>-308 A/A and A/G genotypes to arthritis OR = [2.692 (1.503–4.822, <italic>p</italic> = 0.0007, <italic>p</italic><sub>corr</sub> = 0.0119)] as well as renal SLE manifestation OR = [2.632 (1.575–4.397, <italic>p</italic> = 0.0002, <italic>p</italic><sub>corr</sub> = 0.0034)]. There was a significant association between <italic>TNF-α</italic>-308 A/A and A/G genotypes and the presence of anti-Ro antibodies (Ab) OR = 3.375(1.711–6.658, <italic>p</italic> = 0.0003, <italic>p</italic><sub>corr</sub> = 0.0051). However, the logistic regression analysis revealed that only renal manifestations and the presence of anti-anti-Ro antibodies remained significant after adjustment to the presence of the HLA-DRB1*03:01 haplotype.</p> <p> <italic>Conclusion</italic>. Our studies indicate that the <italic>TNF-α</italic>-308 G/A polymorphism may be a DRB1*03:01 haplotype-dependent genetic risk factor for SLE. However, this SNP was independently associated with renal manifestations and production of anti-Ro Ab.</p> </abstract>